粗糙脉孢菌营养生长周期中的DNA甲基化差异
Differential DNA methylation during the vegetative life cycle of Neurospora crassa.
作者信息
Russell P J, Rodland K D, Rachlin E M, McCloskey J A
出版信息
J Bacteriol. 1987 Jun;169(6):2902-5. doi: 10.1128/jb.169.6.2902-2905.1987.
Abstract
Isotope dilution gas chromatography-mass spectrometry analysis of genomic DNAs isolated from the different growth phases of Neurospora crassa revealed significant differences in the amounts of 5-methylcytosine; the mol % of 5-methylcytosine was 0.36 in conidia (asexual spores), 0.40 in conidial germlings cultured for 3 h, 0.24 in mycelial cells that had grown exponentially for 6 and 12 h, and 0.40 in stationary-phase mycelial cells. These results indicate an approximate inverse correlation between the level of C methylation in the DNA and the presumed level of developmentally related transcriptional activity.
摘要
对从粗糙脉孢菌不同生长阶段分离得到的基因组DNA进行同位素稀释气相色谱-质谱分析，结果显示5-甲基胞嘧啶含量存在显著差异；在分生孢子（无性孢子）中，5-甲基胞嘧啶的摩尔百分比为0.36，在培养3小时的分生孢子萌发体中为0.40，在指数生长6小时和12小时的菌丝体细胞中为0.24，在稳定期菌丝体细胞中为0.40。这些结果表明，DNA中C甲基化水平与推测的发育相关转录活性水平之间大致呈负相关。
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