Dragan A V, Petrushenko O A, Burlak O P, Lukyanetz E A
Fiziol Zh (1994). 2016;62(1):16-24. doi: 10.15407/fz62.01.016.
The activity of TRPA1 and TRPV1 channels, their sensitivity to selective activators - allyl isothiocyanate (AITC) and capsaicin (Caps), especially their interaction were studied. The method of microfluorescent microscopy and Ca2+ sensitive dye fura- 2AM. Registration of changes in the concentration of intracellular Ca2+ was performed by using the ratio of fluorescence signals measured at two wavelengths (R = F1/ F2). Researches were conducted on cultured neurons of rat dorsal ganglia (DRG neurons). Application of AITC and Caps on soma of DRG neurons resulted in an increase in intracellular Ca2+. Consistent repeated Caps applications resulted in a significant reduction in the amplitude of Ca2+ transients ( desensitization of TRPV1 channels), which accounted 20,7% of initial value. Further application of selective TRPA1 channel agonist (AITC) resulted in restoration of sensitivity to capsaicin TRPV1 channels ( resensitization TRPV1 channels). Thus, we have established the presence of regulation of TRPV1 channel activity by TRPA1 channels.
研究了TRPA1和TRPV1通道的活性、它们对选择性激活剂——异硫氰酸烯丙酯（AITC）和辣椒素（Caps）的敏感性，尤其是它们之间的相互作用。采用微荧光显微镜和Ca2+敏感染料fura-2AM的方法。通过测量两个波长下的荧光信号比值（R = F1/F2）来记录细胞内Ca2+浓度的变化。研究在大鼠背根神经节培养神经元（DRG神经元）上进行。将AITC和Caps应用于DRG神经元的胞体导致细胞内Ca2+增加。持续重复应用Caps导致Ca2+瞬变幅度显著降低（TRPV1通道脱敏），占初始值的20.7%。进一步应用选择性TRPA1通道激动剂（AITC）导致TRPV1通道对辣椒素的敏感性恢复（TRPV1通道再敏化）。因此，我们确定了TRPA1通道对TRPV1通道活性的调节作用。
