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阿尔及利亚生乳奶酪中抗葡萄球菌肠毒素生成情况

Anti-Staphylococcal Enterotoxinogenesis of in Algerian Raw Milk Cheese.

作者信息

Tetili Fatiha, Bendali Farida, Perrier Josette, Sadoun Djamila

机构信息

Applied Microbiology Laboratory, Life and Nature Sciences Faculty, Bejaia University, DZ-06000 Bejaia, Algeria.

Paul Cézanne Aix-Marseille III University, Group Molecular Interactions Intestinal Mucosal Microbiote, ISM2-Biosciences UMR CNRS 6263, Faculty of Sciences,
FR-13397 Marseille Cedex 20, France.

出版信息

Food Technol Biotechnol. 2017 Dec;55(4):511-518. doi: 10.17113/ftb.55.04.17.5105.

Abstract

is a potential pathogen contaminating raw milk and dairy products, where it is able to produce thermostable enterotoxins that can cause staphylococcal food poisoning. This study was undertaken to investigate the inhibitory activity of a strain (isolated from milk) on growth and staphylococcal enterotoxin A (SEA) production. In the presence of , the number of the pathogen decreased significantly (p<0.05) after 6 h of incubation in a laboratory medium and milk (3 log CFU/mL reduction compared to pure cultures). SEA concentration was reduced by 79% in the co-cultures. was unable to reach population levels permitting SEA production in the cheese inoculated with during 32 days of storage. In contrast, during the same period, it attained 7 log CFU/g in the cheese manufactured without the lactococcal strain, a level which permitted SEA detection in the cheese extracts. However, this enterotoxin was never detected in the cheese harbouring . These results demonstrate the anti-staphylococcal enterotoxinogenesis potential of the strain and its usefulness in raw milk cheese biopreservation.

摘要

是一种污染生奶和乳制品的潜在病原体,它能够产生可导致葡萄球菌食物中毒的耐热肠毒素。本研究旨在调查一株(从牛奶中分离)对生长和葡萄球菌肠毒素A(SEA)产生的抑制活性。在存在的情况下,在实验室培养基和牛奶中孵育6小时后,病原体数量显著减少(p<0.05)(与纯培养物相比减少3 log CFU/mL)。共培养物中SEA浓度降低了79%。在储存32天期间,接种了的奶酪中无法达到允许SEA产生的菌量水平。相比之下,在同一时期,在没有乳球菌菌株制造的奶酪中它达到了7 log CFU/g,这一水平允许在奶酪提取物中检测到SEA。然而,在含有的奶酪中从未检测到这种肠毒素。这些结果证明了该菌株的抗葡萄球菌产肠毒素潜力及其在生奶奶酪生物保鲜中的有用性。

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