转基因技术在发育过程中研究细胞生物学。
Transgenic Techniques for Investigating Cell Biology During Development.
机构信息
Swarthmore College, Swarthmore, PA, USA.
出版信息
Adv Exp Med Biol. 2018;1029:153-164. doi: 10.1007/978-981-10-7545-2_14.
Abstract
Ascidians are increasingly being used as a system for investigating cell biology during development. The extreme genetic and cellular simplicity of ascidian embryos in combination with superior experimental tractability make this an ideal system for in vivo analysis of dynamic cellular processes. Transgenic approaches to cellular and sub-cellular analysis of ascidian development have begun to yield new insights into the mechanisms regulating developmental signaling and morphogenesis. This chapter focuses on the targeted expression of fusion proteins in ascidian embryos and how this technique is being deployed to garner new insights into the cell biology of development.
摘要
海鞘正越来越多地被用作研究发育过程中细胞生物学的系统。海鞘胚胎在遗传和细胞上的极端简单性，加上出色的实验可操作性，使它们成为分析动态细胞过程的理想系统。对海鞘发育的细胞和亚细胞的转基因分析方法已经开始提供新的见解，揭示了调节发育信号转导和形态发生的机制。本章重点介绍融合蛋白在海鞘胚胎中的靶向表达，以及这项技术如何被用来深入了解发育的细胞生物学。
相似文献
1
Transgenic Techniques for Investigating Cell Biology During Development.
Adv Exp Med Biol. 2018;1029:153-164. doi: 10.1007/978-981-10-7545-2_14.
2
Microinjection of Exogenous DNA into Eggs of Halocynthia roretzi.
Adv Exp Med Biol. 2018;1029:25-35. doi: 10.1007/978-981-10-7545-2_4.
3
The Use of cis-Regulatory DNAs as Molecular Tools.
Adv Exp Med Biol. 2018;1029:49-68. doi: 10.1007/978-981-10-7545-2_6.
4
Practical Guide for Ascidian Microinjection: Phallusia mammillata.
Adv Exp Med Biol. 2018;1029:15-24. doi: 10.1007/978-981-10-7545-2_3.
5
Regulation of synaptotagmin gene expression during ascidian embryogenesis.
Dev Biol. 2002 Apr 15;244(2):293-304. doi: 10.1006/dbio.2002.0584.
6
Roles of Hroth, the ascidian otx gene, in the differentiation of the brain (sensory vesicle) and anterior trunk epidermis in the larval development of Halocynthia roretzi.
Mech Dev. 2004 May;121(5):463-74. doi: 10.1016/j.mod.2004.03.017.
7
Electroporation in Ascidians: History, Theory and Protocols.
Adv Exp Med Biol. 2018;1029:37-48. doi: 10.1007/978-981-10-7545-2_5.
8
The development of three identified motor neurons in the larva of an ascidian, Halocynthia roretzi.
Dev Biol. 2002 Apr 15;244(2):278-92. doi: 10.1006/dbio.2002.0585.
9
Ascidians as a vertebrate-like model organism for physiological studies of Rho GTPase signaling.
Biol Cell. 2003 Jul;95(5):295-302. doi: 10.1016/s0248-4900(03)00052-2.
10
Neural marker genes differently expressed in subsets of embryonic neural cells of the ascidian Halocynthia roretzi.
Zoolog Sci. 2002 Aug;19(8):885-9. doi: 10.2108/zsj.19.885.
引用本文的文献
1
Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.
Biol Open. 2023 Sep 15;12(9). doi: 10.1242/bio.060002. Epub 2023 Sep 5.
2
Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.
bioRxiv. 2023 May 8:2023.05.08.539861. doi: 10.1101/2023.05.08.539861.
3
Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes.
Front Cell Dev Biol. 2020 Oct 20;8:587320. doi: 10.3389/fcell.2020.587320. eCollection 2020.
本文引用的文献
1
Regulated spatial expression of fusion gene constructs with the 5' upstream region of Halocynthia roretzi muscle actin gene in Ciona savignyi embryos.
Rouxs Arch Dev Biol. 1993 Jan;203(1-2):104-112. doi: 10.1007/BF00539896.
2
Nodal and FGF coordinate ascidian neural tube morphogenesis.
Development. 2016 Dec 15;143(24):4665-4675. doi: 10.1242/dev.144733. Epub 2016 Nov 8.
3
as a Simple Chordate Model for Heart Development and Regeneration.
J Cardiovasc Dev Dis. 2016 Sep;3(3). doi: 10.3390/jcdd3030025. Epub 2016 Aug 9.
4
Distinct modes of mitotic spindle orientation align cells in the dorsal midline of ascidian embryos.
Dev Biol. 2015 Dec 1;408(1):66-78. doi: 10.1016/j.ydbio.2015.09.019. Epub 2015 Oct 9.
5
Mitotic Membrane Turnover Coordinates Differential Induction of the Heart Progenitor Lineage.
Dev Cell. 2015 Sep 14;34(5):505-19. doi: 10.1016/j.devcel.2015.07.001. Epub 2015 Aug 20.
6
Centrosomes and spindles in ascidian embryos and eggs.
Methods Cell Biol. 2015;129:317-339. doi: 10.1016/bs.mcb.2015.03.006. Epub 2015 May 27.
7
HaloTag technology: a versatile platform for biomedical applications.
Bioconjug Chem. 2015 Jun 17;26(6):975-86. doi: 10.1021/acs.bioconjchem.5b00191. Epub 2015 May 22.
8
Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine.
Dev Biol. 2015 Jul 1;403(1):43-56. doi: 10.1016/j.ydbio.2015.03.018. Epub 2015 Apr 14.
9
Reciprocal and dynamic polarization of planar cell polarity core components and myosin.
Elife. 2015 Apr 13;4:e05361. doi: 10.7554/eLife.05361.
10
Sequential contraction and exchange of apical junctions drives zippering and neural tube closure in a simple chordate.
Dev Cell. 2015 Jan 26;32(2):241-55. doi: 10.1016/j.devcel.2014.12.017.
Zotero 插件