Radiation Oncology, Stanford University, 300 Pasteur Dr, Stanford, California, USA.
Radiation Monitoring Devices Inc, Watertown, Massachusetts, USA.
Analyst. 2018 Apr 16;143(8):1862-1869. doi: 10.1039/C8AN00106E.
Radioluminescence microscopy is an emerging modality that can be used to image radionuclide probes with micron-scale resolution. This technique is particularly useful as a way to probe the metabolic behavior of single cells and to screen and characterize radiopharmaceuticals, but the quality of the images is critically dependent on the scintillator material used to image the cells. In this paper, we detail the development of a microscopy dish made of a thin-film scintillating material, Lu2O3:Eu, that could be used as the blueprint for a future consumable product. After developing a simple quality control method based on long-lived alpha and beta sources, we characterize the radioluminescence properties of various thin-film scintillator samples. We find consistent performance for most samples, but also identify a few samples that do not meet the specifications, thus stressing the need for routine quality control prior to biological experiments. In addition, we test and quantify the transparency of the material, and demonstrate that transparency correlates with thickness. Finally, we evaluate the biocompatibility of the material and show that the microscopy dish can produce radioluminescent images of live single cells.
放射发光显微镜是一种新兴的模式，可以用于以微米级分辨率成像放射性核素探针。该技术特别适用于探测单个细胞的代谢行为，以及筛选和表征放射性药物，但图像的质量严重依赖于用于成像细胞的闪烁体材料。在本文中，我们详细介绍了一种由薄膜闪烁体材料 Lu2O3:Eu 制成的显微镜培养皿的开发，该培养皿可作为未来消耗品的蓝图。在开发了一种基于长寿命α和β源的简单质量控制方法之后，我们对各种薄膜闪烁体样品的放射发光性能进行了表征。我们发现大多数样品的性能一致，但也发现了一些不符合规格的样品，因此强调在进行生物实验之前需要进行常规的质量控制。此外，我们测试和量化了材料的透明度，并证明透明度与厚度相关。最后，我们评估了材料的生物相容性，并展示了显微镜培养皿可以产生活单个细胞的放射发光图像。
