Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, USA.
Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA.
Stem Cells Transl Med. 2018 Jun;7(6):468-476. doi: 10.1002/sctm.17-0189. Epub 2018 Mar 15.
Hematopoietic stem cells (HSCs) are the self-renewing multipotent progenitors to all blood cell types. Identification and isolation of HSCs for study has depended on the expression of combinations of surface markers on HSCs that reliably distinguish them from other cell types. However, the increasing number of markers required to isolate HSCs has made it tedious, expensive, and difficult for newcomers, suggesting the need for a simpler panel of HSC markers. We previously showed that phenotypic HSCs could be separated based on expression of CD11a and that only the CD11a negative fraction contained true HSCs. Here, we show that CD11a and another HSC marker, endothelial protein C receptor (EPCR), can be used to effectively identify and purify HSCs. We introduce a new two-color HSC sorting method that can highly enrich for HSCs with efficiencies comparable to the gold standard combination of CD150 and CD48. Our results demonstrate that adding CD11a and EPCR to the HSC biologist's toolkit improves the purity of and simplifies isolation of HSCs. Stem Cells Translational Medicine 2018;7:468-476.
造血干细胞（HSCs）是自我更新的多能祖细胞，可以分化为所有类型的血细胞。为了进行研究，鉴定和分离 HSCs 一直依赖于 HSCs 表面标志物的表达组合，这些标志物能够可靠地区分 HSCs 与其他细胞类型。然而，为了分离 HSCs，所需的标志物数量不断增加，这使得这项工作变得繁琐、昂贵，并且对于新手来说也很困难，这表明需要一个更简单的 HSC 标志物面板。我们之前曾表明，可以根据 CD11a 的表达将表型 HSCs 分离出来，并且只有 CD11a 阴性部分才含有真正的 HSCs。在这里，我们表明 CD11a 和另一个 HSC 标志物，内皮蛋白 C 受体（EPCR），可以用于有效鉴定和纯化 HSCs。我们引入了一种新的双色 HSC 分选方法，该方法可以高度富集 HSCs，其效率可与 CD150 和 CD48 的黄金标准组合相媲美。我们的结果表明，将 CD11a 和 EPCR 添加到 HSC 生物学家的工具包中，可以提高 HSCs 的纯度并简化 HSCs 的分离。《干细胞转化医学》2018 年；7:468-476.
