Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Biosens Bioelectron. 2018 Jun 30;109:109-115. doi: 10.1016/j.bios.2018.03.013. Epub 2018 Mar 7.
It is well known that the conventional electrochemiluminescence (ECL) biosensor rely on the heterogeneous assay formats that involves the immobilization of biorecognition probe on the electrode surface before signal collection, which inevitably cause the efficiency of bio-recognition reactions to be limited owing to the existence of local steric hindrance. Herein, a robust, magnetic, and self-accelerated ECL nanosensor based on the multifunctionalized cobalt ferrite magnetite nanoparticles (CoFeO MNPs) was firstly designed for copper ion (Cu) detection in quasi-homogeneous system. The prepared nanosensor has its unique advantages compared to the iron oxide (FeO) MNPs-based nanosensor for which magnetic nanoparticle just provide the reaction interface and magnetic enrichment. Specifically, the prepared CoFeO MNPs-based biosensing platform could bridge the gap between aqueous phase and solid materials in homogeneous solution, achieving the expansion of reaction area and the reduction of local steric hindrance with high biorecognition efficiency. Furthermore, compared with the common magnetite nanosensors, the prepared CoFeO MNPs achieved a set of magnetic collection, biorecognition probes immobilization, rapid separation and signal amplification in an ECL measurement system because it could act as a new co-reaction accelerator in ECL ternary (PTC-NH + SO + CoFeO) system, achieving a self-accelerated biosensing platform with significant enhancement of the detection sensitivity. As expected, the prepared CoFeO MNPs-based ECL nanosensors were successfully applied for ultrasensitive detection of Cuvia click reaction with a linear range from 10 M to 1.0 × 10 M, which exhibited high sensitivity, excellent selectivity and good reproducibility.
众所周知，传统的电化学发光（ECL）生物传感器依赖于异质分析形式，即在信号收集之前将生物识别探针固定在电极表面，这不可避免地导致由于局部空间位阻的存在，生物识别反应的效率受到限制。在此，首次设计了基于多功能化钴铁氧体磁铁矿纳米粒子（CoFeO MNPs）的稳健、磁性和自加速 ECL 纳米传感器，用于准均相体系中铜离子（Cu）的检测。与基于氧化铁（FeO）MNPs 的纳米传感器相比，所制备的纳米传感器具有独特的优势，因为磁性纳米颗粒仅提供反应界面和磁富集。具体而言，所制备的基于 CoFeO MNPs 的生物传感平台可以在均相溶液中桥接水相和固相之间的间隙，实现反应面积的扩展和局部空间位阻的降低，具有较高的生物识别效率。此外，与常见的磁铁矿纳米传感器相比，所制备的 CoFeO MNPs 在 ECL 三组分（PTC-NH + SO + CoFeO）系统中充当新的共反应加速剂，实现了自加速生物传感平台，极大地提高了检测灵敏度。不出所料，所制备的基于 CoFeO MNPs 的 ECL 纳米传感器成功应用于 Cuvia 点击反应的超灵敏检测，线性范围从 10 M 到 1.0×10 M，表现出高灵敏度、优异的选择性和良好的重现性。
