Division of Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, India.
Central Instrumental Facility, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
Plant Physiol Biochem. 2018 Jun;127:1-10. doi: 10.1016/j.plaphy.2018.03.008. Epub 2018 Mar 7.
Development of flower color in plants is a complex process. Among others, it is an important trait for ornamental flowering plants. Canna is a flowering ornamental plant of family Cannaceae. To understand the molecular mechanism of flower color development in Canna, RNA sequencing from flower tissues of two contrasting flower color cultivars, Red President (RP) and Tropical Sunrise (TS) was performed. More than 27.0 million and 19.0 million clean reads were obtained from RP and TS, respectively. The combined clean reads were assembled into 147,295 unigenes. The Canna unigenes showed maximum homology with Populus trichocarpa (26.79%). A total of 2702 unigenes expressed differentially between the two cultivars of which 1972 were up-regulated and 730 were down-regulated in RP. Phenylpropanoid and flavonoid biosynthetic processes were the significant processes in RP. Expression of a vast number of transcription factors including MYB, bHLH, ARF, and WRKY were higher in RP than TS. The expression analysis of RNA sequencing data was validated by qRT-PCR analysis. Further, concentration of measured anthocyanidins and flavonols were very low or absent in TS, corroborating largely with our transcriptome data. These findings may help in understanding flower color development in Canna and in future crop breeding program.
植物花色的形成是一个复杂的过程。花色是观赏花卉植物的一个重要特征。美人蕉是美人蕉科的一种开花观赏植物。为了了解美人蕉花色形成的分子机制，对两个具有不同花色的品种，红总统（RP）和热带日出（TS）的花组织进行了 RNA 测序。从 RP 和 TS 中分别获得了超过 2700 万和 1900 万条清洁读数。组合清洁读数被组装成 147295 个 unigenes。美人蕉 unigenes 与毛白杨（Populus trichocarpa）的同源性最高（26.79%）。在两个品种之间表达差异的 unigenes 共有 2702 个，其中 RP 中有 1972 个上调，730 个下调。苯丙素和类黄酮生物合成过程是 RP 中的显著过程。大量转录因子如 MYB、bHLH、ARF 和 WRKY 的表达在 RP 中高于 TS。RNA 测序数据的表达分析通过 qRT-PCR 分析进行了验证。此外，TS 中测量的花色素苷和类黄酮的浓度非常低或不存在，这与我们的转录组数据基本一致。这些发现可能有助于理解美人蕉的花色形成，并在未来的作物育种计划中发挥作用。
