来自肌浆网的（Ca2++Mg2+）-ATP酶中异硫氰酸荧光素结合位点与位于膜上的荧光探针之间的距离。
Distances between the FITC-binding site in the (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum and fluorescent probes located in the membrane.
作者信息
Teruel J A, Gómez-Fernández J C
出版信息
Biochem Int. 1987 Mar;14(3):409-16.
PMID:2954553
Abstract
Fluorescence energy transfer measurements have been performed to investigate the distances between the FITC-binding site in the (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum and 8-aniline-1-naftalenesulfonate (ANS-) and diphenylhexatriene (DPH) localized in different sites of the membrane. The distances calculated between FITC bound to the ATPase and ANS- and DPH in the membrane, were approx. 51 A and 60 A, respectively.
摘要
已进行荧光能量转移测量，以研究肌浆网（Ca2++Mg2+）-ATP酶中FITC结合位点与定位在膜不同部位的8-苯胺-1-萘磺酸盐（ANS-）和二苯基己三烯（DPH）之间的距离。计算得出，与ATP酶结合的FITC与膜中的ANS-和DPH之间的距离分别约为51 Å和60 Å。
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