[α,β-亚甲基ADP与乙酰胆碱对新生犬离体胰腺胰岛素分泌的增效协同作用]

[Potentiating synergism between alpha, beta-methylene ADP and acetylcholine on the secretion of insulin from the isolated pancreas of the newborn dog].

作者信息

Bertrand G, Gross R, Chapal J, Loubatières-Mariani M M

出版信息

C R Seances Soc Biol Fil. 1987;181(1):35-9.

Abstract

A possible interaction between alpha,beta-methylene ADP, a stable analogue of ADP, and acetylcholine, was studied on insulin secretion from isolated new-born dog pancreas, perfused in the presence of a non stimulating glucose concentration (4.2 mM). alpha,beta-methylene ADP alone (16.5 microM) and acetylcholine alone (0.5 microM) induced a comparable increment of insulin secretion. When the two substances were simultaneously infused, the increment of secretion was significantly higher than the sum of the increments induced by alpha,beta-methylene ADP and acetylcholine infused separately (p less than 0.005). Our results show a potentiating synergism between alpha,beta-methylene ADP and acetylcholine, on insulin secretion, alpha,beta-methylene ADP acting via a P2 purinoceptor and acetylcholine via a muscarinic receptor. Similar results were previously obtained on the rat pancreas.

摘要

在非刺激葡萄糖浓度（4.2 mM）存在的情况下，对灌注的新生犬离体胰腺胰岛素分泌进行研究，以探讨α,β-亚甲基ADP（一种ADP的稳定类似物）与乙酰胆碱之间可能的相互作用。单独使用α,β-亚甲基ADP（16.5微摩尔）和单独使用乙酰胆碱（0.5微摩尔）均可引起胰岛素分泌相当程度的增加。当同时注入这两种物质时，分泌增加显著高于分别注入α,β-亚甲基ADP和乙酰胆碱所引起的增加之和（p小于0.005）。我们的结果表明，α,β-亚甲基ADP和乙酰胆碱在胰岛素分泌上具有增强协同作用，α,β-亚甲基ADP通过P2嘌呤受体起作用，乙酰胆碱通过毒蕈碱受体起作用。先前在大鼠胰腺上也获得了类似结果。