Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.
Department of Analytical Research & Development, Amgen Inc., 1 Amgen Center Drive, Thousand Oaks, CA, 91320, USA.
J Am Soc Mass Spectrom. 2018 Jul;29(7):1339-1344. doi: 10.1007/s13361-018-1923-0. Epub 2018 Mar 15.
Proof of concept evidence is presented for a new method for the determination of isoaspartate, an important post-translational modification. Chemical derivatization is performed using common reagents for the modification of carboxylic acids and shown to yield suitable diagnostic information with regard to isomerization at the aspartate residue. The diagnostic gas phase chemistry is probed by collision-induced dissociation mass spectrometry, on the timescale of the MS experiment and semi-quantitative calibration of the percentage of isoaspartate in a peptide sample is demonstrated. Graphical Abstract ᅟ.
提出了一种新的测定异天冬氨酸（一种重要的翻译后修饰）的方法的概念验证证据。使用羧酸修饰的常用试剂进行化学衍生化，并显示出在天冬氨酸残基异构化方面具有合适的诊断信息。通过碰撞诱导解离质谱探测到诊断气相化学，并在 MS 实验的时间尺度上进行了研究，同时还证明了对肽样品中异天冬氨酸百分比进行半定量校准的可行性。图摘要。
