Ichinohasama Reo, Nishiguchi Koji M, Fujita Kosuke, Aizawa Naoko, Inoue Takashi, Sasaki Erika, Kunikata Hiroshi, Nakazawa Toru
a Department of Ophthalmology , Tohoku University Graduate School of Medicine , Sendai , Japan.
b Department of Advanced Ophthalmic Medicine , Tohoku University Graduate School of Medicine , Sendai , Japan.
Curr Eye Res. 2018 Jun;43(6):804-809. doi: 10.1080/02713683.2018.1451544. Epub 2018 Mar 16.
The purpose of the study is to investigate the correlation between intraocular anti-retinal antibodies and clinical measurements in patients with rhegmatogenous retinal detachment (RRD) and proliferative vitreoretinopathy (PVR).
Aqueous humor and vitreous samples were collected from patients with RRD, PVR, and from control subjects with macular hole. The levels of total protein (TP), IgG, and anti-retinal antibodies were determined with a bicinchoninic acid assay, enzyme-linked immunosorbent assay, and dot blot, respectively. Correlations between these measurements were assessed using Pearson's correlation test. Analysis of variance followed by a post-hoc test or the Student t-test was used to compare differences between groups.
The levels of anti-retinal antibodies and IgG were correlated with each other (P < 0.010). The IgG concentration was higher in patients with PVR than in controls in both the aqueous humor (P < 0.001) and the vitreous (P < 0.001), but not in patients with RRD. Conversely, TP levels and anti-retinal antibodies in both ocular fluids from RRD and PVR patients did not significantly differ from the controls. In a subgroup analysis, vitreal anti-retinal antibody levels were correlated with average macular thickness in the re-attached macula following surgery for macula-off RRD/PVR (P = 0.012). Furthermore, patients with post-operative cystoid macular edema had a higher level of vitreal anti-retinal antibodies than those without (P = 0.009).
Intravitreal anti-retinal antibodies were increased in the eyes with maculopathy after surgical intervention for RRD/PVR.
本研究旨在调查孔源性视网膜脱离（RRD）和增殖性玻璃体视网膜病变（PVR）患者眼内抗视网膜抗体与临床测量指标之间的相关性。
收集RRD、PVR患者以及黄斑裂孔对照受试者的房水和玻璃体样本。分别采用二喹啉甲酸测定法、酶联免疫吸附测定法和斑点印迹法测定总蛋白（TP）、IgG和抗视网膜抗体水平。使用Pearson相关检验评估这些测量指标之间的相关性。采用方差分析及事后检验或Student t检验比较组间差异。
抗视网膜抗体水平与IgG水平相互相关（P < 0.010）。PVR患者房水（P < 0.001）和玻璃体（P < 0.001）中的IgG浓度均高于对照组，但RRD患者并非如此。相反，RRD和PVR患者两种眼液中的TP水平和抗视网膜抗体与对照组相比无显著差异。在亚组分析中，黄斑脱离的RRD/PVR手术后，玻璃体抗视网膜抗体水平与复位黄斑的平均黄斑厚度相关（P = 0.012）。此外，术后发生黄斑囊样水肿的患者玻璃体抗视网膜抗体水平高于未发生者（P = 0.009）。
RRD/PVR手术干预后，黄斑病变眼内玻璃体抗视网膜抗体增加。
