Germann T, Huhn H, Zimmermann F, Rüde E
Eur J Immunol. 1987 Jun;17(6):775-81. doi: 10.1002/eji.1830170607.
The data presented in this report describe an antigen-independent activation pathway leading to reinduction of proliferation of class II major histocompatibility complex (MHC)-restricted murine T cell lines that after previous antigen-specific stimulation reverted to a resting state. Antigen-independent proliferation and interleukin 2 (IL2)-receptor expression occur in the presence of splenic accessory cells, exogenous IL2 and a soluble factor(s) provisionally termed T cell-stimulating factor(s) (TSF). Each of these components is essential for inducing growth. TSF is found in the supernatant of an autoreactive T cell line upon stimulation with syngeneic accessory cells. Neither TSF nor accessory cells can be replaced by IL1 and by some other cytokines. Monoclonal antibodies against class II MHC molecules, the T cell receptor and L3T4 do not block this antigen-independent stimulation. This demonstrates that the function of the accessory cell in this system is not MHC restricted and that the T cell receptor is also not involved. Furthermore, it is suggested that the blocking of L3T4 molecules by antibody will mediate a negative signal only if T cells are triggered via their antigen receptors.
本报告中呈现的数据描述了一种抗原非依赖性激活途径，该途径可导致II类主要组织相容性复合体（MHC）限制的鼠T细胞系再次增殖，这些T细胞系在先前的抗原特异性刺激后恢复到静止状态。抗原非依赖性增殖和白细胞介素2（IL2）受体表达在脾辅助细胞、外源性IL2和一种暂时称为T细胞刺激因子（TSF）的可溶性因子存在的情况下发生。这些成分中的每一种对于诱导生长都是必不可少的。TSF存在于同基因辅助细胞刺激后自身反应性T细胞系的上清液中。TSF和辅助细胞都不能被IL1和其他一些细胞因子替代。针对II类MHC分子、T细胞受体和L3T4的单克隆抗体不会阻断这种抗原非依赖性刺激。这表明该系统中辅助细胞的功能不受MHC限制，并且T细胞受体也不参与其中。此外，有人提出，只有当T细胞通过其抗原受体被触发时，抗体对L3T4分子的阻断才会介导负信号。
