产蛋鸡的昼夜钙喂养模式与锌浓度、生物钟基因表达、钙转运体和氧化状态有关。
Circadian calcium feeding regime in laying hens related to zinc concentration, gene expression of circadian clock, calcium transporters and oxidative status.
机构信息
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; Hunan Co-Innovation Center of Safety Animal Production, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China.
出版信息
J Trace Elem Med Biol. 2018 Dec;50:518-526. doi: 10.1016/j.jtemb.2018.03.002. Epub 2018 Mar 8.
The study was conducted to investigate the effects of different circadian calcium feeding regimes on parameters of Zn status and gene expression of circadian clock, calcium transporters and oxidative status in laying hens. In total, 180 of 41-weeks Brown Hy-line laying hens were assigned randomly into three groups, 1-CON group (Control Ca, diets contained 3.4% Ca at both 0730 and 1530 h), 2-HL group (High-low Ca, diets contained 3.6%-3.2% Ca respectively) and 3-LH group (Low-high Ca, diets contained 3.2%-3.6% Ca respectively), which were fed a certain amount of control diet at 0730 h and 1530 h. Blood, tibia, jejunum and kidney samples were collected at 4 h intervals with initial starting at 0800 h after 10 weeks of experiment. Compared with the CON group: 1) the serum zinc in HL group increased at 2000 h, but lower at 1600 h in LH group (P < 0.05). 2) in jejunum, circadian clock genes including CLOCK and BMAL1 expression of HL group were down-regulated at 0000 h and 1600 h, as well as CLOCK, BMAL1, Cry2, Per3 and calcium transporter gene NCX1 in LH group at 2000 h (P < 0.05). 3) in kidney, CLOCK, Cry1, Cry2 and Per3 of LH group were up-regulated at 0400 h, CLOCK at 0000 h as well, while CLOCK at 2000 h were down-regulated (P < 0.05). 4) in kidney, the calcium transporters including PMCA, CaBP and CA of LH group were up-regulated at 0000 h, but PMCA and CaBP of LH group were down-regulated at 0800 h, 1200 h and 1600 h, as well as CA at 1600 h and PMCA at 2000 h of LH group (P < 0.05), and the oxidative gene SOD of the LH group was up-regulated at 0400 h, as well as CAT at 0400 h, SOD and GPX1 at 1200 h in HL group, but SOD at 1600 h and 2000 h, and GPX1 at 1600 h were down-regulated in LH group (P < 0.05). These results demonstrated that the dynamic circadian calcium feeding regime affected circadian rhythms of serum zinc concentration as well as the expression of certain genes related to the circadian clock, calcium transport and antioxidative capacity, and circadian calcium feeding regimes may therefore be considered with regard to improving the calcium usability.
这项研究旨在探讨不同的昼夜钙喂养方案对产蛋鸡锌状态参数和昼夜节律时钟、钙转运体和氧化状态相关基因表达的影响。总共将 180 只 41 周龄的布朗海兰产蛋鸡随机分为三组，1-CON 组（对照钙，在 0730 和 1530 时分别含有 3.4%的钙）、2-HL 组（高低钙，日粮分别含有 3.6%-3.2%的钙）和 3-LH 组（低高钙，日粮分别含有 3.2%-3.6%的钙），在 0730 时喂食一定量的对照饲料和 1530 时。实验进行 10 周后，每隔 4 小时采集一次血液、胫骨、空肠和肾脏样本，起始时间为 0800 时。与 CON 组相比：1）HL 组的血清锌在 2000 时增加，但 LH 组在 1600 时较低（P<0.05）。2）在空肠中，HL 组的昼夜节律时钟基因 CLOCK 和 BMAL1 的表达在 0000 时和 1600 时下调，以及 LH 组的 CLOCK、BMAL1、Cry2、Per3 和钙转运体基因 NCX1 在 2000 时下调（P<0.05）。3）在肾脏中，LH 组的 CLOCK、Cry1、Cry2 和 Per3 在 0400 时上调，CLOCK 在 0000 时也上调，而 CLOCK 在 2000 时下调（P<0.05）。4）在肾脏中，LH 组的钙转运体包括 PMCA、CaBP 和 CA 在 0000 时上调，但 PMCA 和 CaBP 在 LH 组在 0800 时、1200 时和 1600 时下调，以及 CA 在 1600 时和 PMCA 在 2000 时下调（P<0.05），而 LH 组的氧化基因 SOD 在 0400 时上调，以及 CAT 在 0400 时、SOD 和 GPX1 在 1200 时上调，但 SOD 在 1600 时和 2000 时以及 GPX1 在 1600 时下调（P<0.05）。这些结果表明，动态昼夜钙喂养方案影响血清锌浓度的昼夜节律以及与昼夜节律时钟、钙转运和抗氧化能力相关的某些基因的表达，因此昼夜钙喂养方案可能被认为是提高钙利用率的一种方法。
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