一种用于体内DNA克隆的cos - mini - Mu载体。

A cos-mini-Mu vector for in vivo DNA cloning.

作者信息

Gramajo H C, de Mendoza D

出版信息

Gene. 1987;51(1):85-90. doi: 10.1016/0378-1119(87)90477-x.

Abstract

Construction of a mini-Mu plasmid vector containing a cosmid replicon is described. Upon derepression of mini-Mu transposition, bacterial DNA sequences can be flanked by the integrated mini-Mu. These sequences can then be packaged into lambda heads by superinfection with a lambda helper phage. Cosmid clones carrying particular bacterial genes can be recovered by selection after infection of appropriate strains with the cosmid transducing lambda lysate. We report here the successful in vivo cloning of several Escherichia coli genes using the transposoncosmid vector.

摘要

本文描述了一种含有黏粒复制子的微型 Mu 质粒载体的构建方法。在微型 Mu 转座去阻遏后，细菌 DNA 序列可被整合的微型 Mu 侧翼包围。然后，通过用 λ 辅助噬菌体超感染，这些序列可被包装到 λ 头部。在用黏粒转导 λ 裂解物感染合适菌株后，通过选择可回收携带特定细菌基因的黏粒克隆。我们在此报告了使用转座子 - 黏粒载体成功地在体内克隆了几个大肠杆菌基因。