来自海洋细菌格氏假单胞菌KMM 9500的含5-乙酰氨基-3,5-二脱氧-L-甘油-L-甘露糖壬-2-酮糖酸的O-多糖。
5-Acetamido-3,5-dideoxy-L-glycero-L-manno-non-2-ulosonic acid-containing O-polysaccharide from marine bacterium Pseudomonas glareae KMM 9500.
作者信息
Kokoulin Maxim S, Kalinovsky Anatoly I, Romanenko Lyudmila A, Mikhailov Valery V
机构信息
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, Vladivostok, 690022, Russia.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, Vladivostok, 690022, Russia.
出版信息
Carbohydr Res. 2018 May 22;461:19-24. doi: 10.1016/j.carres.2018.03.004. Epub 2018 Mar 8.
The O-polysaccharide was isolated from the lipopolysaccharide of a marine bacterium Pseudomonas glareae KMM 9500 and studied by chemical methods along with 1D and 2D H and C NMR spectroscopy including H,H-TOCSY, H,H-COSY, H,H-ROESY, H,C-HSQC and H,C-HMBC experiments. The O-polysaccharide was found to consist of linear tetrasaccharide repeating units constituted by D-glucuronic acid (D-GlcA), L-rhamnose (L-Rha), D-glucose (D-Glc) and 5-acetamido-7,9-O-[(S)-1-carboxyethylidene]-3,5-dideoxy-L-glycero-L-manno-non-2-ulosonic acid (Sug7,9(S-Pyr)), partially O-acetylated at position 8 (∼70%): →4)-α-D-GlcpA-(1→3)-β-L-Rhap-(1→4)-β-D-Glcp-(1→4)-β-Sugp8Ac(∼70%)7,9(S-Pyr)-(2→.
从海洋细菌格氏假单胞菌KMM 9500的脂多糖中分离出O-多糖，并采用化学方法以及一维和二维氢谱和碳谱核磁共振波谱（包括氢-氢全相关谱、氢-氢化学位移相关谱、氢-氢旋转坐标系下核Overhauser效应谱、氢-碳异核单量子相干谱和氢-碳异核多键相关谱实验）对其进行研究。发现该O-多糖由线性四糖重复单元组成，这些单元由D-葡萄糖醛酸（D-GlcA）、L-鼠李糖（L-Rha）、D-葡萄糖（D-Glc）和5-乙酰氨基-7,9-O-[(S)-1-羧基亚乙基]-3,5-二脱氧-L-甘油-L-甘露糖-2-酮糖酸（Sug7,9(S-Pyr)）构成，在8位部分O-乙酰化（约70%）：→4)-α-D-葡萄糖醛酸-(1→3)-β-L-鼠李糖-(1→4)-β-D-葡萄糖-(1→4)-β-Sugp8Ac(约70%)7,9(S-Pyr)-(2→ 。
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