State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China.
College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, 050024, Hebei, China.
J Pharm Biomed Anal. 2018 May 30;154:207-215. doi: 10.1016/j.jpba.2018.03.005. Epub 2018 Mar 9.
Platycladi Cacumen (PC) is a traditional Chinese medicine used for the treatment of hemorrhages, cough, asthma and hair loss. To get a better understanding of the chemical constituents in PC, ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) and diagnostic ion filtering strategy were firstly employed for chemical profiling of PC. A total of 43 compounds including organic acids and derivatives, flavonoids as well as phenylpropanolds were unambiguously or reasonably identified. Coumarin and lignan were reported for the first time in PC. Chemical variation of 39 batches of PC from different geographical origins and 10 batches of processed product of PC was subsequently investigated by quantitation of nine major flavonoids. The results determined by UPLC coupled with diode array detection (UPLC-DAD) and hierarchical cluster analysis (HCA) indicated that the contents of flavonoids in PC samples differ greatly. This work provides an efficient approach to comprehensively evaluate the quality of PC.
柏子仁为传统中药，具有治疗出血、咳嗽、哮喘和脱发等功效。为了更好地了解柏子仁中的化学成分，首次采用超高效液相色谱-电喷雾电离四极杆飞行时间串联质谱联用（UPLC-Q-TOF-MS/MS）和诊断离子过滤策略对柏子仁进行化学分析。共鉴定出 43 种化合物，包括有机酸及其衍生物、黄酮类化合物和苯丙醇类化合物。香豆素和木脂素类化合物首次在柏子仁中被发现。通过对 39 批不同产地和 10 批炮制柏子仁中 9 种主要黄酮类化合物的定量分析，进一步研究了其化学变异性。结果表明，通过超高效液相色谱-二极管阵列检测（UPLC-DAD）和层次聚类分析（HCA）所确定的柏子仁样品中黄酮类化合物的含量差异很大。本研究为全面评价柏子仁的质量提供了一种有效的方法。
