N-(p-coumaroyl) serotonin induces cell cycle arrest and apoptosis in breast cancer cells.
作者信息
Mitsis Michail, Alexiou George A, Vartholomatos Evrysthenis, Markopoulos Georgios, Lazari Diamanto, Hodaj Entela, Nastos Dimitrios, Zagorianakou Panagiota, Galani Vasiliki, Kyritsis Athanasios P
机构信息
Department of Surgery, Ioannina University Hospital & University of Ioannina, Ioannina, Greece.
出版信息
J BUON. 2018 Jan-Feb;23(1):129-133.
PURPOSE
Breast cancer is the most commonly diagnosed malignancy among women. Breast cancer cells may develop resistance to current chemotherapy, thus new chemotherapeutic agents are urgently needed.
METHODS
A major number of drugs with anticancer activity have been isolated from plants. Herewith, we investigated for the first time the effect of N-(p-coumaroyl) serotonin (CS), isolated from Centaurea seed on a drug-resistant breast carcinoma (MCF-7) cells. Viability and proliferation of the cells were examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Caspace-8, cell cycle, and CD24/CD44/CD56/ CD58/CD71/CD15 expression were tested with flow cytometry.
RESULTS
Treatment with CS significantly reduced cell viability. Induction of cell death and cell cycle arrest was confirmed with flow cytometry. After treatment with CS, there was a dose-dependent decrease in CD24/CD44/CD58/CD71 expression, whereas there was no change in CD56 and CD15 expression.
CONCLUSION
The treatment of breast cancer cells with CS may represent a novel therapeutic strategy and requires further investigation.
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