Kang Chuan-Zhi, Jiang Jing-Yi, Yang Wan-Zhen, Zhou Li, Lv Chao-Geng, Li Jia-Xing, Wang Sheng, Zhou Tao, Yang Ye, Huang Lu-Qi, Guo Lan-Ping
State Key Laboratory and Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Guiyang College of Traditional Chinese Medicine, Guiyang 550025, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jan;43(2):248-253. doi: 10.19540/j.cnki.cjcmm.20171023.003.
Studies on the characteristic chemical markers of sulfur fumigation have become an effective auxiliary way for quality control of traditional Chinese medicine. This study established a quantitative analysis method for the determination of -hydroxybenzyl hydrogen sulfite (-HS) in Gastrodiae Rhizoma (GR) based on UPLC-MS/MS. Then, 37 batches of GR were screened, and the results showed that 27 batches of them were sulfur-fumigated. Especially, the GR samples in Yunnan producing areas were sulfur-fumigated more seriously. Based on the stability of -HS after different storage time and heat treatment methods, it was found that the content of -HS was stable and reliable in the storage period of 8 months and under normal heat treatment (50, 60, 70, 80 °C) conditions. In conclusion, this study shows a high sensitivity, good selectivity and good stability of -HS, which can provide reference for the quality control and sulfur fumigation screening of GR.
对硫熏特征化学标志物的研究已成为中药质量控制的一种有效辅助手段。本研究基于超高效液相色谱-串联质谱法（UPLC-MS/MS）建立了天麻中对羟基苄基亚硫酸氢酯（-HS）的定量分析方法。随后，对37批次天麻进行筛选，结果显示其中27批次为硫熏天麻。特别是云南产区的天麻样品硫熏更为严重。基于-HS在不同储存时间和热处理方式后的稳定性，发现其在8个月储存期及常规热处理（50、60、70、80℃）条件下含量稳定可靠。综上所述，本研究表明-HS具有高灵敏度、良好的选择性和稳定性，可为天麻的质量控制和硫熏筛选提供参考。
