Kang Chuan-Zhi, Yang Wan-Zhen, Zhou Li, Jiang Jing-Yi, Lv Chao-Geng, Wang Sheng, Zhou Tao, Yang Ye, Huang Lu-Qi, Guo Lan-Ping
State Key Laboratory and Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Guiyang College of Traditional Chinese Medicine, Guiyang 550025, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jan;43(2):254-260. doi: 10.19540/j.cnki.cjcmm.20171023.009.
As Gastrodiae Rhizoma (GR) is one of the herbs more seriously affected by sulfur fumigation, so its quality has been always of a great concern. In this paper, GR samples collected from eight main producing areas and in three forms were fumigated with sulfur and quantitatively and qualitatively analyzed based on UPLC-Q-TOF-MS/MS. The results showed that the contents of gastrodin, parishin, parishin B and parishin C were decreased, while the content of parishin E was increased after sulfur fumigation treatment. Besides, a new sulfur marker named p-hydroxybenzyl hydrogen sulfite was produced in sulfur-fumigated GR samples. As compared with producing origins, forms had a greater impact on the quality of GR, especially in Hongtianma and Wutianma. Besides, the contents of gastrodins and parishins in Hongtianma from Jilin were lowest as compared with those in other producing areas. This might be correlated with planting patterns and environmental factors. In conclusion, sulfur fumigation has a more obvious impact on the quality of GR than origins and forms, which is attributed to the generation of new sulfur fumigated markers.
天麻是受硫磺熏蒸影响较为严重的药材之一，因此其质量一直备受关注。本文对采自八个主要产地、三种形态的天麻样品进行硫磺熏蒸，并基于超高效液相色谱-四极杆-飞行时间串联质谱（UPLC-Q-TOF-MS/MS）进行定量和定性分析。结果表明，硫磺熏蒸处理后，天麻素、派立辛、派立辛B和派立辛C的含量降低，而派立辛E的含量增加。此外，硫磺熏蒸的天麻样品中产生了一种名为对羟基苄基亚硫酸氢酯的新的硫磺标记物。与产地相比，形态对天麻质量的影响更大，尤其是红天麻和乌天麻。此外，吉林红天麻中天麻素和派立辛的含量与其他产地相比最低。这可能与种植模式和环境因素有关。综上所述，硫磺熏蒸对天麻质量的影响比产地和形态更为明显，这归因于新的硫磺熏蒸标记物的产生。
