λ噬菌体中转录抗终止所需位点nutR的分析。
Analysis of nutR, a site required for transcription antitermination in phage lambda.
作者信息
Zuber M, Patterson T A, Court D L
出版信息
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4514-8. doi: 10.1073/pnas.84.13.4514.
Abstract
Deletions extending from the cro gene into boxA and nutR of the Rho-dependent tR1 terminator of bacteriophage lambda have been generated and cloned between promoters and the galK gene of Escherichia coli on a multicopy plasmid. Terminators placed between the promoters and galK restrict transcription and expression of galK on these plasmids. However, when lambda N protein is provided, and if a functional N interaction site, nutR, is intact, transcription antitermination occurs and galK expression increases. Deletions into the nutR region affect the ability to antiterminate. From the results obtained we conclude that: boxA, a site believed to bind host factors (Nus), is not required for transcription antitermination in this system; the host NusA function is required even in the absence of boxA; nutR is required for N antitermination; translation across the nutR sequence prevents N-dependent antitermination.
摘要
已经构建了从噬菌体λ的Rho依赖性tR1终止子的cro基因延伸至boxA和nutR的缺失，并将其克隆到多拷贝质粒上大肠杆菌的启动子与galK基因之间。置于启动子和galK之间的终止子会限制这些质粒上galK的转录和表达。然而，当提供λ N蛋白时，并且如果功能性的N相互作用位点nutR完整无损，就会发生转录抗终止，galK表达增加。进入nutR区域的缺失会影响抗终止能力。根据所得结果我们得出结论：boxA，一个被认为结合宿主因子（Nus）的位点，在该系统中对于转录抗终止不是必需的；即使在没有boxA的情况下，宿主NusA功能也是必需的；nutR对于N抗终止是必需的；跨越nutR序列的翻译会阻止N依赖性抗终止。
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