Institute of Legal Medicine, Hannover Medical School (MHH), Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 1;1084:1-3. doi: 10.1016/j.jchromb.2018.03.027. Epub 2018 Mar 15.
Multiple analyte adduct formation was examined and discussed in the context of reproducible signal detection in liquid chromatography-tandem mass spectrometry applied in the analysis of biologically-related samples. Appropriate infusion solutions were prepared in HO/methanol (3/97, v/v) with 1 mM sodium acetate and 10 mM acetic acid. An API 4000 QTrap tandem mass spectrometer was used for experiments performed in the negative scan mode (-Q1 MS) and the negative enhanced product ion mode (-EPI). γ‑Hydroxybutyrate and its deuterated form were used as model compounds to highlight both the complexity of adduct formation in popular mobile phases used and the effective signal compensation by the application of isotope-labelled analytes as internal standards.
多分析物加合物的形成在液相色谱-串联质谱分析生物相关样品中重现性信号检测的背景下进行了研究和讨论。在 HO/甲醇（3/97，v/v）中制备了含有 1mM 醋酸钠和 10mM 乙酸的适当的进样溶液。采用 API 4000 QTrap 串联质谱仪在负扫描模式（-Q1 MS）和负增强产物离子模式（-EPI）下进行实验。γ-羟基丁酸及其氘代形式被用作模型化合物，以突出在常用流动相下加合物形成的复杂性，以及应用同位素标记分析物作为内标进行有效信号补偿。
