Mebrahtu Y, Beach R F, Khamala C P, Hendricks L D
Trans R Soc Trop Med Hyg. 1986;80(6):914-22. doi: 10.1016/0035-9203(86)90258-0.
Isoenzyme characterization of the Simulium damnosum Theobald sibling species complex from two widely separated geographical areas in Kenya is presented based on 10 enzymatic loci. Four river systems in Western and Nyanza Provinces, namely, the Yala, Lusumu, Isiukhu and the Nzoia harbouring S. damnosum s.l. were compared among themselves and with S. damnosum s.l. collected from the Thiba and the Nyamindi river systems in the Mt. Kenya area. The two populations were easily separable using PGM, HK and, more than 73% of the time, with PGI. Using the first two enzymatic loci, PGM and HK, all the western Kenya S. damnosum s.l. belong to the same population while those from Mt. Kenya areas belong to a different population. In both geographical zones there was less than 20% qualitative and quantitative polymorphism within infraspecific forms in any given breeding area of S. damnosum s.l. Three enzymes, ME, XDH, and G-6-PDH had isomorphic mobilities for both the Mt. Kenya and western Kenya populations. Four other enzyme/substrate systems tested had no satisfactory resolution as a diagnostic value.
基于10个酶位点，对来自肯尼亚两个相距甚远地理区域的黑足蚋（Simulium damnosum Theobald）同胞物种复合体进行了同工酶特征分析。对西省和尼扬扎省的4条河流系统，即亚拉河、卢苏木河、伊苏胡河和恩佐亚河（其中栖息着黑足蚋指名亚种）进行了相互比较，并与从肯尼亚山地区的蒂巴河和尼亚明迪河系统采集的黑足蚋指名亚种进行了比较。使用磷酸葡萄糖变位酶（PGM）、己糖激酶（HK），以及在超过73%的情况下使用磷酸葡萄糖异构酶（PGI），可以轻松区分这两个种群。利用前两个酶位点，即PGM和HK，肯尼亚西部的所有黑足蚋指名亚种都属于同一个种群，而来自肯尼亚山地区的则属于不同种群。在这两个地理区域中，在黑足蚋指名亚种的任何给定繁殖区域内，种下形态内的定性和定量多态性均低于20%。对于肯尼亚山和肯尼亚西部的种群，三种酶，即苹果酸酶（ME）、黄嘌呤脱氢酶（XDH）和葡萄糖-6-磷酸脱氢酶（G-6-PDH）具有同构迁移率。测试的其他四个酶/底物系统作为诊断价值没有令人满意的分辨率。
