Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
Heart Center & Beijing Key Laboratory of Hypertension Research, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
J Cardiovasc Transl Res. 2019 Feb;12(1):75-83. doi: 10.1007/s12265-017-9784-7. Epub 2018 Mar 20.
The role and miRNA expression profile of exosomes in hypertension remain largely unknown. Therefore, next-generation sequencing was used to define the miRNA expression profile of plasma exosomes in spontaneously hypertensive rats (SHRs), the most widely used animal model of human essential hypertension, and their controls, normotensive Wistar-Kyoto rats (WKYs). Results revealed that percentages of miRNA in the total small RNA isolated from SHRs and WKYs were not significantly different. Twenty-seven miRNAs were significantly differentially expressed (DE) between SHR and WKY exosomes, including 23 up-regulated and four down-regulated in SHR exosomes as compared to WKY exosomes. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis of top 10 DE miRNAs identified hypertension-specific target genes/signaling pathways. In conclusion, our findings indicated the selective packing of miRNA cargo into exosomes under hypertensive status, which could facilitate the development of potential targets for the diagnosis, prevention, and treatment of hypertension.
外泌体在高血压中的作用和 miRNA 表达谱在很大程度上尚不清楚。因此，我们使用下一代测序来定义自发性高血压大鼠（SHR）——最常用于人类原发性高血压的动物模型——及其对照品（正常血压的 Wistar-Kyoto 大鼠，WKY）的血浆外泌体中的 miRNA 表达谱。结果表明，从 SHR 和 WKY 外泌体中分离出的总 small RNA 中 miRNA 的百分比没有显著差异。在 SHR 外泌体与 WKY 外泌体之间有 27 个 miRNA 显著差异表达（DE），其中 23 个在 SHR 外泌体中上调，4 个下调。对 top 10 DE miRNAs 的基因本体论分析和京都基因与基因组百科全书通路分析鉴定了高血压特异性靶基因/信号通路。总之，我们的研究结果表明，在高血压状态下，miRNA 货物被选择性地包装到外泌体中，这可能有助于为高血压的诊断、预防和治疗开发潜在的靶标。
