Key Laboratory of Functional Polymer Materials Ministry of Education, College of Chemistry , Nankai University , Tianjin 300071 , China.
J Phys Chem B. 2018 Apr 12;122(14):3900-3907. doi: 10.1021/acs.jpcb.8b01447. Epub 2018 Mar 22.
Synthesis and self-assembly of bioconjugates composed of proteins and synthetic molecules have been widely studied because of the potential applications in medicine, biotechnology, and nanotechnology. One of the challenging research studies in this area is to develop organic solvent-free approaches to the synthesis and self-assembly of amphiphilic bioconjugates. In this research, dialysis-assisted approach, a method based on unimer-aggregate equilibrium, was applied in the coassembly of lysozyme and conjugate of cholesterol and glutathione (Ch-GSH). In phosphate buffer solution, amphiphilic Ch-GSH conjugate self-assembles into vesicles, and the vesicle solution is dialyzed against lysozyme solution. Negatively charged Ch-GSH unimers produced in the unimer-vesicle exchange equilibrium, diffuse across the dialysis membrane and have electrostatic interaction with positively charged lysozyme, resulting in the formation of Ch-GSH-lysozyme bioconjugate. Above a critical concentration, the three-component bioconjugate molecules self-assemble into bioactive vesicles.
由于在医学、生物技术和纳米技术方面的潜在应用，由蛋白质和合成分子组成的生物缀合物的合成和自组装得到了广泛的研究。该领域具有挑战性的研究之一是开发无有机溶剂的方法来合成和自组装两亲性生物缀合物。在这项研究中，应用透析辅助方法（一种基于单体-聚集体平衡的方法）来共组装溶菌酶和胆固醇与谷胱甘肽的轭合物（Ch-GSH）。在磷酸盐缓冲溶液中，两亲性 Ch-GSH 轭合物自组装成囊泡，囊泡溶液用溶菌酶溶液透析。单体-囊泡交换平衡中产生的带负电荷的 Ch-GSH 单体扩散穿过透析膜，并与带正电荷的溶菌酶发生静电相互作用，导致 Ch-GSH-溶菌酶生物缀合物的形成。在超过临界浓度时，三组分生物缀合物分子自组装成生物活性囊泡。
