Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary; Doctoral School of Biology, University of Szeged, H-6726 Szeged, Hungary.
Center for Gene Research, Nagoya University, Furocyo, Chikusa, Nagoya 464-8607, Japan.
J Plant Physiol. 2018 Apr;223:96-104. doi: 10.1016/j.jplph.2018.02.008. Epub 2018 Mar 7.
Phosphatidylglycerol is an essential phospholipid for photosynthesis and other cellular processes. We investigated the role of phosphatidylglycerol in cell division and metabolism in a phophatidylglycerol-auxotrophic strain of Synechococcus PCC7942. Here we show that phosphatidylglycerol is essential for the photosynthetic electron transfer and for the oligomerisation of the photosynthetic complexes, notably, we revealed that this lipid is important for non-linear electron transport. Furthermore, we demonstrate that phosphatidylglycerol starvation elevated the expressions of proteins of nitrogen and carbon metabolism. Moreover, we show that phosphatidylglycerol-deficient cells changed the morphology, became elongated, the FtsZ ring did not assemble correctly, and subsequently the division was hindered. However, supplementation with phosphatidylglycerol restored the ring-like structure at the mid-cell region and the normal cell size, demonstrating the phosphatidylglycerol is needed for normal septum formation. Taken together, central roles of phosphatidylglycerol were revealed; it is implicated in the photosynthetic activity, the metabolism and the fission of bacteria.
磷脂酰甘油是光合作用和其他细胞过程的必需磷脂。我们研究了在磷酯酰甘油营养缺陷型的聚球藻 PCC7942 菌株中，磷脂酰甘油在细胞分裂和代谢中的作用。在这里，我们表明磷脂酰甘油对于光合作用的电子传递和光合复合物的寡聚化是必需的，特别是，我们揭示了这种脂质对于非线性电子传递很重要。此外，我们证明磷酯酰甘油饥饿会提高氮和碳代谢的蛋白质的表达。此外，我们还表明，缺乏磷酯酰甘油的细胞改变了形态，变得细长，FtsZ 环不能正确组装，随后分裂受阻。然而，用磷酯酰甘油补充可以恢复在细胞中部的环状结构和正常的细胞大小，表明磷酯酰甘油对于正常的隔膜形成是必需的。总之，揭示了磷脂酰甘油的核心作用；它参与了细菌的光合作用、代谢和分裂。
