Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
Biochem Biophys Res Commun. 2018 May 5;499(2):209-214. doi: 10.1016/j.bbrc.2018.03.128. Epub 2018 Mar 22.
Tissue closure involves the coordinated unidirectional movement of a group of cells without loss of cell-cell contact. However, the molecular mechanisms controlling the tissue closure are not fully understood. Here, we demonstrate that Lamin C, the sole A-type lamin in Drosophila, contributes to the process of thorax closure in pupa. High expression of Lamin C was observed at the leading front of the migrating wing imaginal discs. Live imaging analysis revealed that knockdown of Lamin C in the thorax region affected the coordinated movement of the leading front, resulting in incomplete tissue fusion required for formation of the adult thorax. The closure defect due to knockdown of Lamin C correlated with insufficient accumulation of F-actin at the front. Our study indicates a link between A-type lamin and the cell migration behavior during tissue closure.
组织闭合涉及一组细胞的协调单向运动，而不会失去细胞间的接触。然而，控制组织闭合的分子机制尚不完全清楚。在这里，我们证明了果蝇中唯一的 A 型层粘连蛋白 lamin C 有助于蛹期胸部闭合过程。在迁移的翅膀 imaginal discs 的前缘观察到 lamin C 的高表达。实时成像分析显示，胸部区域 lamin C 的敲低会影响前缘的协调运动，导致形成成虫胸部所需的不完全组织融合。由于 lamin C 的敲低导致的闭合缺陷与前缘处 F-actin 的积累不足有关。我们的研究表明 A 型层粘连蛋白与组织闭合过程中的细胞迁移行为之间存在联系。
