从伊朗克尔曼的人类、小牛和山羊中分离出的产志贺毒素菌株的血清群、亚型和毒力因子。
Serogroups, subtypes and virulence factors of shiga toxin-producing isolated from human, calves and goats in Kerman, Iran.
作者信息
Taghadosi Rohollah, Shakibaie Mohammad Reza, Alizade Hesam, Hosseini-Nave Hossein, Askari Asma, Ghanbarpour Reza
机构信息
Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran.
Infectious and Tropical Disease Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2018 Winter;11(1):60-67.
AIM
The present study was conducted to detect the occurrence, serogroups, virulence genes and phylogenetic relationship of shiga toxin-producing (STEC) in human, clave and goat in Kerman (southeast of Iran).
BACKGROUND
STEC have emerged as the important foodborne zoonotic pathogens causing human gastrointestinal disease and confirming the risk to public health.
METHODS
A total of 671 fecal samples were collected from diarrheic patients (n=395) and healthy calves (n=156) and goats (n=120) and screened for the presence of gene. Furthermore, the prevalence of and variants, serotypes (O157, O145, O103, O26, O111, O91, O128, and O45), phylogenetic groups and the presence of , , , and virulence genes were studied.
RESULTS
Prevalence of STEC in human diarrheic isolates was 1.3% (5 isolates), in claves was 26.3% (41 isolates) and in goats was 27.5% (33 isolates). gene was the most prevalent variant and detected in 75 isolates. Furthermore, was the most predominant subtype, found in 56 isolates. The identified in 36 (45.6%) isolates, followed by 5 (6.3%), 4 (5.1%), 2 (2.5%) and 2 (2.5%). Most of the isolates belonged to phylogroup B1. Only two O26 and one O91 isolates were detected in our study.
CONCLUSION
Our results show that STEC strains were widespread among healthy domestic animals in the southeast of Iran.
目的
本研究旨在检测伊朗东南部克尔曼地区人类、小牛和山羊中产志贺毒素大肠杆菌（STEC）的发生情况、血清群、毒力基因及系统发育关系。
背景
STEC已成为引起人类胃肠道疾病的重要食源性人畜共患病原体，对公众健康构成风险。
方法
共收集了671份粪便样本，其中腹泻患者样本395份、健康小牛样本156份和山羊样本120份，对其进行基因筛查。此外，还研究了和变体的流行情况、血清型（O157、O145、O103、O26、O111、O91、O128和O45）、系统发育群以及、、、和毒力基因的存在情况。
结果
人类腹泻分离株中STEC的流行率为1.3%（5株），小牛中为26.3%（41株），山羊中为27.5%（33株）。基因是最常见的变体，在75株分离株中被检测到。此外，是最主要的亚型，在56株分离株中被发现。在36株（45.6%）分离株中鉴定出，其次是5株（6.3%）、4株（5.1%）、2株（2.5%）和2株（2.5%）。大多数分离株属于B1系统发育群。在我们的研究中仅检测到2株O26和1株O91分离株。
结论
我们的结果表明，STEC菌株在伊朗东南部的健康家畜中广泛存在。
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