小鼠基因分型

Mouse Genotyping.

作者信息

Vuong Sandra, Delgado-Olguin Paul

机构信息

Translational Medicine, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G0A4, Canada.

Department of Molecular Genetics, University of Toronto, 1 King's College Cir, Toronto, ON, M5S 1A8, Canada.

出版信息

Methods Mol Biol. 2018;1752:1-9. doi: 10.1007/978-1-4939-7714-7_1.

DOI:10.1007/978-1-4939-7714-7_1

PMID:29564757

Abstract

Genotyping is an invaluable tool for identifying organisms carrying genetic variations including insertions and deletions. This method involves the extraction of DNA from animal tissue samples and subsequent amplification of genomic regions of interest by polymerase chain reaction (PCR). The amplified products are analyzed by agarose gel electrophoresis based on their size.

摘要

基因分型是一种用于识别携带包括插入和缺失在内的基因变异的生物体的宝贵工具。该方法包括从动物组织样本中提取DNA，随后通过聚合酶链反应（PCR）扩增感兴趣的基因组区域。扩增产物根据其大小通过琼脂糖凝胶电泳进行分析。