小鼠中从视杆细胞到视杆双极细胞突触传递的体外功能评估
Ex Vivo Functional Evaluation of Synaptic Transmission from Rods to Rod Bipolar Cells in Mice.
作者信息
Pahlberg Johan, Majumder Anurima, Artemyev Nikolai O
机构信息
Department of Ophthalmology, Stein Eye Institute, UCLA, Los Angeles, CA, USA.
Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA.
出版信息
Methods Mol Biol. 2018;1753:203-216. doi: 10.1007/978-1-4939-7720-8_14.
Abstract
Mice have been widely used as a model organism to study mechanisms of phototransduction and synaptic transmission in the retina. Genetic manipulations and electrophysiological techniques for analysis of photoreceptor and rod bipolar cell function in mice are uniquely advanced. Here, we describe a set of biochemical and electrophysiological techniques for evaluation of synaptic transmission at the rod-rod bipolar cell synapse, which represents the first and key step in the processing of dim-light visual information.
摘要
小鼠已被广泛用作模式生物，以研究视网膜中的光转导和突触传递机制。用于分析小鼠光感受器和视杆双极细胞功能的基因操作和电生理技术非常先进。在这里，我们描述了一套用于评估视杆-视杆双极细胞突触处突触传递的生化和电生理技术，这是暗光视觉信息处理的第一步也是关键步骤。
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