Ames A, Nesbett F B
J Neurochem. 1981 Oct;37(4):867-77. doi: 10.1111/j.1471-4159.1981.tb04473.x.
Methods are described for isolating adult rabbit retinal and maintaining it in a medium designed to resemble CSF. Morphologic, metabolic, nd electrophysiologic measurements obtained on the in vitro retinas showed that they remained in a nearly physiological state for at least 8 h, and even after 2 days in vitro they still exhibited a high level of metabolic activity and electrical responsiveness to light. Physiological activity was modified by photic stimulation, and data are presented to document changes in metabolism in response to the changes in function. The isolated retina appears to offer a number of unusual advantages for studying relationships between function and metabolism in organized mammalian central nervous tissue.
本文描述了分离成年兔视网膜并将其维持在一种模拟脑脊液的培养基中的方法。对体外培养的视网膜进行的形态学、代谢和电生理测量表明，它们在至少8小时内保持接近生理状态，即使在体外培养2天后，它们仍表现出高水平的代谢活性和对光的电反应性。生理活动通过光刺激进行调节，并提供数据以记录功能变化时代谢的变化。分离的视网膜似乎为研究有组织的哺乳动物中枢神经组织中功能与代谢之间的关系提供了许多独特的优势。
