Rutgers, The State University of New Jersey, Department of Biomedical Engineering, Piscataway, New J, United States.
Rutgers, The State University of New Jersey, Department of Chemical and Biochemical Engineering, Pis, United States.
J Biomed Opt. 2018 Mar;23(3):1-4. doi: 10.1117/1.JBO.23.3.030505.
Rare-earth-doped nanocomposites have appealing optical properties for use as biomedical contrast agents, but few systems exist for imaging these materials. We describe the design and characterization of (i) a preclinical system for whole animal in vivo imaging and (ii) an integrated optical coherence tomography/confocal microscopy system for high-resolution imaging of ex vivo tissues. We demonstrate these systems by administering erbium-doped nanocomposites to a murine model of metastatic breast cancer. Short-wave infrared emissions were detected in vivo and in whole organ imaging ex vivo. Visible upconversion emissions and tissue autofluorescence were imaged in biopsy specimens, alongside optical coherence tomography imaging of tissue microstructure. We anticipate that this work will provide guidance for researchers seeking to image these nanomaterials across a wide range of biological models.
稀土掺杂纳米复合材料具有作为生物医学对比剂的诱人光学特性，但用于这些材料成像的系统很少。我们描述了（i）用于小动物体内成像的临床前系统和（ii）用于离体组织高分辨率成像的集成光相干断层扫描/共聚焦显微镜系统的设计和表征。我们通过将掺铒纳米复合材料施用于转移性乳腺癌的小鼠模型来演示这些系统。在体内和整个器官的离体成像中检测到短波长红外发射。在活检标本中，与组织微观结构的光相干断层扫描成像一起，对可见上转换发射和组织自体荧光进行成像。我们预计这项工作将为寻求在广泛的生物模型中对这些纳米材料进行成像的研究人员提供指导。
