Duchenne 肌营养不良症患者尿液中 miRNA-29、-23 和 -21 的下调。
Downregulation of miRNA-29, -23 and -21 in urine of Duchenne muscular dystrophy patients.
机构信息
The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child Health, 30 Guildford Street, London WC1N 1EH, UK.
School of Pharmacy & Biomedical Sciences, University of Portsmouth, St Michael's Building, Portsmouth PO1 2DT, UK.
出版信息
Epigenomics. 2018 Jul;10(7):875-889. doi: 10.2217/epi-2018-0022. Epub 2018 Mar 22.
AIM
To study the signature of 87 urinary miRNAs in Duchenne muscular dystrophy (DMD) patients, select the most dysregulated and determine statistically significant differences in their expression between controls, ambulant (A) and nonambulant (NA) DMD patients, and patients on different corticosteroid regimens. Patients/materials & methods: Urine was collected from control (n = 20), A (n = 31) and NA (n = 23) DMD patients. miRNA expression was measured by reverse transcription-quantitative PCR.
RESULTS
miR-29c-3p was significantly downregulated in A DMD patients while miR-23b-3p and miR-21-5p were significantly downregulated in NA DMD patients compared with age-matched controls.
CONCLUSION
miR-29c-3p, miR-23b-3p and miR-21-5p are promising novel noninvasive biomarkers for DMD, and miR-29c-3p levels are differentially affected by different steroid regimens, supporting the antifibrotic effect of steroid therapy.
目的
研究 87 种尿 microRNA 在杜氏肌营养不良症（DMD）患者中的特征，选择最失调的 microRNA，并确定其在对照组、能走动（A）和不能走动（NA）DMD 患者以及不同皮质类固醇治疗方案患者中的表达存在统计学显著差异。
患者/材料与方法：收集 20 名对照者、31 名 A 组和 23 名 NA 组 DMD 患者的尿液。采用逆转录定量 PCR 法测量 microRNA 表达。
结果
与年龄匹配的对照组相比，A 组 DMD 患者的 miR-29c-3p 明显下调，而 NA 组 DMD 患者的 miR-23b-3p 和 miR-21-5p 明显下调。
结论
miR-29c-3p、miR-23b-3p 和 miR-21-5p 是 DMD 有前途的新型非侵入性生物标志物，miR-29c-3p 水平受不同类固醇治疗方案的影响不同，支持类固醇治疗的抗纤维化作用。
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