Department of Otolaryngology, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, 712000, Shaanxi, China.
Department of Neurology, Yang Ling Demonstration Zone Hospital, Yangling, 712100, Shaanxi, China.
Appl Biochem Biotechnol. 2024 Jan;196(1):275-295. doi: 10.1007/s12010-023-04558-y. Epub 2023 Apr 29.
This study aims to investigate the mechanism of tumor-derived exosomal (EVs) SNHG16 in promoting the progression of nasopharyngeal carcinoma (NPC). QRT-PCR was used to detect the expression of SNHG16, miR-23b-5p and MCM6 in NPC. MTT, flow cytometry and transwell were used to detect the effects of them on the proliferation, cycle, apoptosis and invasion ability of NPC. Transmission electron microscopy, Western blotting and BCA were used to verify the regulation of exosome secretion under different oxygen environments. Our results showed that hypoxia induces tumor-derived exosome SNHG16 to mediate NPC progression through the miR-23b-5p/MCM6 pathway.
本研究旨在探讨肿瘤衍生的外泌体（EVs）SNHG16 在促进鼻咽癌（NPC）进展中的机制。实时荧光定量 PCR（QRT-PCR）用于检测 NPC 中 SNHG16、miR-23b-5p 和 MCM6 的表达。MTT、流式细胞术和 Transwell 用于检测它们对 NPC 增殖、周期、凋亡和侵袭能力的影响。透射电子显微镜、Western blot 和 BCA 用于验证不同氧环境下外泌体分泌的调节。我们的结果表明，缺氧诱导肿瘤衍生的外泌体 SNHG16 通过 miR-23b-5p/MCM6 通路介导 NPC 的进展。
