Jiao Jiao, Lu Yu-Zhi, Xia Ni, Wang Yi-Qiu, Tang Ting-Ting, Nie Shao-Fang, Lv Bing-Jie, Wang Ke-Jing, Wen Shuang, Li Jing-Yong, Zhou Xing-Di, Liao Yu-Hua, Cheng Xiang
Cell Physiol Biochem. 2018;46(1):23-35. doi: 10.1159/000488405. Epub 2018 Mar 20.
BACKGROUND/AIMS: Newly identified IL-10-producing regulatory B cells (Bregs) have been shown to play an important role in the suppression of immune responses. Chronic immune activation participates in the pathogenesis of dilated cardiomyopathy (DCM) but whether Bregs are involved in its development remains unclear. We aimed to investigate the circulating frequency and function of Bregs in DCM.
In total, 35 DCM patients (20 men and 15 women) and 44 healthy controls (23 men and 21 women) were included in the experiment, and the frequency of Bregs was detected using flow cytometry.
According to our results, the frequency of circulating IL-10-producing Bregs was significantly lower in DCM patients compared with healthy controls. Furthermore, the CD24hiCD27+ B cell subset in which IL-10-producing Bregs were mainly enriched from DCM patients showed impaired IL-10 expression and a decreased ability to suppress the TNF-α production of CD4+CD25- Tconv cells and to maintain Tregs differentiation. Correlation analysis showed that the frequency of IL-10-producing Bregs and the suppressive function of CD24hiCD27+ B cells were positively correlated with left ventricular ejection fraction and negatively correlated with NT-proBNP in DCM patients.
In conclusion, the reduced frequency and impaired functions suggest a potential role of Bregs in the development of DCM.
背景/目的：新发现的产生白细胞介素-10的调节性B细胞（Bregs）已被证明在免疫反应抑制中起重要作用。慢性免疫激活参与扩张型心肌病（DCM）的发病机制，但Bregs是否参与其发展仍不清楚。我们旨在研究DCM中Bregs的循环频率和功能。
总共35例DCM患者（20例男性和15例女性）和44例健康对照者（23例男性和21例女性）纳入实验，使用流式细胞术检测Bregs的频率。
根据我们的结果，与健康对照者相比，DCM患者中循环产生白细胞介素-10的Bregs频率显著降低。此外，DCM患者中主要富集产生白细胞介素-10的Bregs的CD24hiCD27+ B细胞亚群显示白细胞介素-10表达受损，抑制CD4+CD25-Tconv细胞产生肿瘤坏死因子-α以及维持调节性T细胞分化的能力下降。相关性分析显示，DCM患者中产生白细胞介素-10的Bregs频率和CD24hiCD27+ B细胞的抑制功能与左心室射血分数呈正相关，与N末端脑钠肽前体呈负相关。
总之，频率降低和功能受损表明Bregs在DCM发展中具有潜在作用。
