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转录组分析揭示超显性在烟草杂种优势中起关键作用。

Transcriptomic analysis reveals overdominance playing a critical role in nicotine heterosis in Nicotiana tabacum L.

机构信息

Key Laboratory of Tobacco Quality in Guizhou province, Guizhou University, Guiyang, 550025, China.

College of Tobacco, Guizhou University, Guiyang, 550025, China.

出版信息

BMC Plant Biol. 2018 Mar 22;18(1):48. doi: 10.1186/s12870-018-1257-x.

DOI:10.1186/s12870-018-1257-x
PMID:29566653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5863848/
Abstract

BACKGROUND

As a unique biological phenomenon, heterosis has been concerned with the superior performance of the heterosis than either parents. Despite several F1 hybrids, containing supernal nicotine content, had been discovered and applied to heterosis utilization in Nicotiana tabacum L., nevertheless, the potential molecular mechanism revealing nicotine heterosis has not been illustrated clearly.

RESULT

Phenotypically, the F1 hybrids (Vall6 × Basma) show prominent heterosis in nicotine content by 3 years of field experiments. Transcriptome analysis revealed that genes participating in nicotine anabolism (ADC, PMT, MPO, QPT, AO, QS, QPT, A622, BBLs) and nicotine transport (JAT2, MATE1 and 2, NUP1 and 2) showed an upregulated expression in the hybrid, a majority of which demonstrated an overdominant performance. RT-PCR confirmed that nicotine anabolism was induced in the hybrid.

CONCLUSIONS

These findings strongly suggest that nicotine synthesis and transport efficiency improved in hybrid and overdominance at gene-expression level played a critical role in heterosis of nicotine metabolism.

摘要

背景

杂种优势是一种独特的生物学现象，其杂种表现优于双亲。尽管已经发现并应用了几种含有超高尼古丁含量的 F1 杂种，用于烟草杂种优势利用，但揭示尼古丁杂种优势的潜在分子机制尚不清楚。

结果

表型上，杂种（Vall6×Basma）在田间试验的 3 年内表现出尼古丁含量的显著杂种优势。转录组分析表明，参与尼古丁生物合成的基因（ADC、PMT、MPO、QPT、AO、QS、QPT、A622、BBLs）和尼古丁转运（JAT2、MATE1 和 2、NUP1 和 2）在杂种中表达上调，其中大多数表现出超显性表现。RT-PCR 证实杂种中诱导了尼古丁生物合成。

结论

这些发现强烈表明，杂种中尼古丁的合成和转运效率提高，基因表达水平的超显性在尼古丁代谢杂种优势中起着关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/b1fa1364a4ec/12870_2018_1257_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/4b81ba3ff353/12870_2018_1257_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/953c29650a11/12870_2018_1257_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/b1fa1364a4ec/12870_2018_1257_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/43368d82fa21/12870_2018_1257_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/4172d6604ed0/12870_2018_1257_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/f6124facfeed/12870_2018_1257_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/cd9cf7b49d96/12870_2018_1257_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/4b81ba3ff353/12870_2018_1257_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/953c29650a11/12870_2018_1257_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91a/5863848/b1fa1364a4ec/12870_2018_1257_Fig7_HTML.jpg

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