Park Yun-Gwi, Lee Seung-Eun, Son Yeo-Jin, Jeong Sang-Gi, Shin Min-Young, Kim Won-Jae, Kim Eun-Young, Park Se-Pill
Stem Cell Research Center, Jeju National University, 102 Jejudaehak-ro, Jeju, Jeju Special Self-Governing Province, 63243, Korea.
Reprod Fertil Dev. 2018 Aug;30(9):1204-1213. doi: 10.1071/RD17444.
Oxidative stress is partly responsible for the poor quality of IVM oocytes. The present study investigated the effects of the antioxidant β-cryptoxanthin on the IVM of porcine oocytes and the in vitro development of the ensuing embryos. Oocytes were matured in IVM medium containing different concentrations of β-cryptoxanthin (0, 0.1, 1, 10 or 100μM). Treatment with 1µM β-cryptoxanthin (Group 1B) improved polar body extrusion and the expression of maturation-related genes in cumulus cells and oocytes compared with control. In addition, levels of reactive oxygen species decreased significantly in Group 1B, whereas there were significant increases in glutathione levels and expression of the antioxidant genes superoxide dismutase 1 and peroxiredoxin 5 in this group. After parthenogenetic activation, although the cleavage rate did not differ between the control and 1B groups, the blastocyst formation rate was higher in the latter. Moreover, the total number of cells per blastocyst and relative mRNA levels of pluripotency marker and antioxidant genes were significantly higher in the 1B compared with control group. These results demonstrate that β-cryptoxanthin decreases oxidative stress in porcine oocytes and improves their quality and developmental potential.
氧化应激是导致体外成熟（IVM）卵母细胞质量不佳的部分原因。本研究调查了抗氧化剂β-隐黄质对猪卵母细胞体外成熟及后续胚胎体外发育的影响。卵母细胞在含有不同浓度β-隐黄质（0、0.1、1、10或100μM）的体外成熟培养基中成熟。与对照组相比，用1μMβ-隐黄质处理（1B组）可改善卵丘细胞和卵母细胞中极体排出及成熟相关基因的表达。此外，1B组中活性氧水平显著降低，而该组中谷胱甘肽水平以及抗氧化基因超氧化物歧化酶1和过氧化物酶5的表达显著增加。孤雌激活后，虽然对照组和1B组之间的分裂率没有差异，但后者的囊胚形成率更高。此外，与对照组相比，1B组每个囊胚的细胞总数以及多能性标记物和抗氧化基因的相对mRNA水平显著更高。这些结果表明，β-隐黄质可降低猪卵母细胞中的氧化应激，并提高其质量和发育潜力。
