Fırat Fatih, Erdemir Fikret, Kölükçü Engin, Gevrek Fikret, Benli İsmail, Ünsal Velid
Department of Urology, Tokat State Hospital, Tokat-Turkey.
Ulus Travma Acil Cerrahi Derg. 2018 Mar;24(2):89-96. doi: 10.5505/tjtes.2017.25730.
We aimed to demonstrate the effectiveness of oxytocin on the testes for treating ischemia-reperfusion injury.
A total of 24 male Wistar albino rats weighing 250-320 g were used. The rats were randomized into three groups of eight rats. Group 1 was assessed as the control group. In Group 2 rats, testicular torsion was first performed, followed by testicular detorsion to induce reperfusion injury. In Group 3, following testicular torsion and detorsion, oxytocin was administered before inducing reperfusion. Testicular tissues were histologically evaluated, spermatogenic parameters were assessed using the Johnsen scoring system, and the mean Johnsen score was calculated.
Histological tests revealed significantly different results between the testicular torsion group and the oxytocin-treated torsion and control groups as well as between the oxytocin-treated torsion group and the control and testicular torsion groups (p=0.010 and 0.012, respectively). Biochemical test results revealed that superoxide dismutase and glutathione peroxidase levels were significantly lower in Group 2 than in Group 1 (p=0.007 and 0.007, respectively). Malondialdehyde and nitric oxide levels were significantly lower in Group 3 than in Group 2 (p=0.017 and 0.014, respectively).
These results indicate that oxytocin can be considered as an alternative agent for treating testicular torsion in clinical practice to minimize tissue damage.
我们旨在证明催产素对睾丸治疗缺血再灌注损伤的有效性。
总共使用了24只体重250 - 320克的雄性Wistar白化大鼠。大鼠被随机分为三组，每组8只。第1组作为对照组。在第2组大鼠中，先进行睾丸扭转，然后进行睾丸复位以诱导再灌注损伤。在第3组中，在睾丸扭转和复位后，在诱导再灌注前给予催产素。对睾丸组织进行组织学评估，使用约翰森评分系统评估生精参数，并计算平均约翰森评分。
组织学测试显示，睾丸扭转组与催产素治疗的扭转组和对照组之间以及催产素治疗的扭转组与对照组和睾丸扭转组之间存在显著不同的结果（分别为p = 0.010和0.012）。生化测试结果显示，第2组中超氧化物歧化酶和谷胱甘肽过氧化物酶水平显著低于第1组（分别为p = 0.007和0.007）。第3组中丙二醛和一氧化氮水平显著低于第2组（分别为p = 0.017和0.014）。
这些结果表明，在临床实践中，催产素可被视为治疗睾丸扭转以尽量减少组织损伤的替代药物。
