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关于电脉冲传递在生物样本电穿孔中应用的报告的推荐意见和要求。

Recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples.

机构信息

Department of Experimental Oncology, Institute of Oncology, Ljubljana, Zaloska 2, 1000 Ljubljana, Slovenia; Faculty of Health Sciences, University of Primorska, Polje, 42, 6310 Izola, Slovenia.

Department of Experimental Oncology, Institute of Oncology, Ljubljana, Zaloska 2, 1000 Ljubljana, Slovenia.

出版信息

Bioelectrochemistry. 2018 Aug;122:69-76. doi: 10.1016/j.bioelechem.2018.03.005. Epub 2018 Mar 12.

Abstract

Electric field-induced membrane changes are an important approach in the life sciences. However, the developments in knowledge and translational applications face problems of reproducibility. Indeed, a quick survey of the literature reveals a lack of transparent and comprehensive reporting of essential technical information in many papers. Too many of the published scientific papers do not contain sufficient information for proper assessment of the presented results. The general rule/guidance in reporting experimental data should require details on exposure conditions such that other researchers are able to evaluate, judge and reproduce the experiments and data obtained. To enhance dissemination of information and reproducibility of protocols, it is important to agree upon nomenclature and reach a consensus on documentation of experimental methods and procedures. This paper offers recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples in life science.

摘要

电场诱导的膜变化是生命科学中的一个重要方法。然而,知识的发展和转化应用面临着可重复性的问题。事实上,快速查阅文献就会发现,许多论文都缺乏对基本技术信息的透明和全面报告。发表的科学论文中有太多的论文没有包含足够的信息,无法对所呈现的结果进行适当的评估。报告实验数据的一般规则/指南应该要求详细说明暴露条件,以便其他研究人员能够评估、判断和再现所获得的实验和数据。为了增强信息的传播和协议的可重复性,就应用电脉冲传递技术对生物样本进行电穿孔而言,为报告的内容命名并就实验方法和程序的文件记录达成共识非常重要。本文为生命科学中电脉冲传递应用于生物样品电穿孔的报告提供了建议和要求。

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