School of Environmental Science and Engineering, Shandong University, Jinan 250000, PR China.; Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China.
Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China.
J Photochem Photobiol B. 2018 Apr;181:164-169. doi: 10.1016/j.jphotobiol.2018.03.014. Epub 2018 Mar 15.
Attached microalgae cultivation for the algae-based products is considered as a promising approach to simplify biomass recovery processes and reduce the cost. However, as an incipient research field, biomass accumulation is the mainly index for attached microalgal growth evaluation. To break through such limitations, physiological properties of attached microalgae (e.g. the oxygen evolving activity and the main organic composition of cells), which are important for microalgal growth evaluation but are still unclear in most studies, were studied using an attached microalgae culture system, i.e. suspended-solid phase photobioreactor (ssPBR) in this paper. As light, nutrients and other environmental conditions of attached microalgae were different from the suspended microalgae, physiological properties of attached microalgae also varied from the suspended ones. Besides the relatively lower biomass accumulation rate, attached microalgae also had a lower oxygen evolving activity (65% on average) comparing to suspended microalgae. The composition of microalgae changed towards accumulating more protein when suspended microalgae turned to attached status. The relative protein content of attached microalgae (50.1% ± 10.1%) was approximately 30% higher than the suspended algae (36.0% ± 16.1%) on average. The discovery of physiological properties of attached microalgae in this paper could help the production of high-protein microalgae-related products and explain some phenomenon during the production of microalgae-related products.
附着微藻培养被认为是简化生物质回收过程和降低成本的有前途的方法。然而，作为一个新兴的研究领域，生物质积累是附着微藻生长评价的主要指标。为了突破这些限制，本文使用附着微藻培养系统（即悬浮固相反光生物反应器（ssPBR））研究了附着微藻的生理特性（例如，氧气产生活性和细胞的主要有机组成），这些特性对于微藻生长评价很重要，但在大多数研究中仍不清楚。与悬浮微藻相比，附着微藻的光照、营养和其他环境条件不同，其生理特性也不同。除了较低的生物质积累率外，附着微藻的氧气产生活性（平均为 65%）也较低。当悬浮微藻转变为附着状态时，微藻的组成向积累更多蛋白质的方向变化。附着微藻的相对蛋白质含量（50.1%±10.1%）比悬浮藻类（36.0%±16.1%）平均高约 30%。本文对附着微藻生理特性的发现有助于生产高蛋白微藻相关产品，并解释微藻相关产品生产过程中的一些现象。
