Institute of Biology Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.
Food Chem. 2018 Jul 30;255:97-103. doi: 10.1016/j.foodchem.2018.02.053. Epub 2018 Feb 12.
Reverse micelles (RMs) as nanocarriers of nisin were optimized for the highest water and bacteriocin content. RMs formulated with either refined olive oil or sunflower oil, distilled monoglycerides, ethanol, and water were effectively designed. Structural characterization of the RMs was assessed using Electron Paramagnetic Resonance Spectroscopy and Small Angle X-ray Scattering in the presence and absence of nisin. No conformational changes occurred in the presence of nisin for the nanocarriers. To assess efficacy of the loaded systems, their antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes was tested in lettuce leaves and minced meat, respectively. Antimicrobial activity was evident in both cases. Interestingly, a synergistic antimicrobial effect was observed in lettuce leaves and to a lesser extent in minced meat between nisin and some of the nanocarriers' constituents (probably ethanol). Our findings suggest complex interactions that take place when RMs are applied in different food matrices.
反胶束（RMs）作为乳链菌肽的纳米载体，其优化的重点是最高的水和抑菌素含量。采用精制橄榄油或葵花籽油、蒸馏单甘油脂、乙醇和水来有效设计 RMs。使用电子顺磁共振波谱和小角度 X 射线散射技术在有或没有乳链菌肽的情况下对 RMs 的结构特征进行评估。在纳米载体中存在乳链菌肽的情况下，没有发生构象变化。为了评估负载系统的功效，分别在生菜叶和碎肉中测试了负载系统对金黄色葡萄球菌和单核细胞增生李斯特菌的抗菌活性。在这两种情况下都表现出了抗菌活性。有趣的是，在生菜叶中和在碎肉中，乳链菌肽和一些纳米载体成分（可能是乙醇）之间观察到协同抗菌作用。我们的研究结果表明，当 RMs 应用于不同的食物基质时，会发生复杂的相互作用。
