Food Science and Human Nutrition Department, Institute of Food and Agriculture Science, University of Florida, Gainesville, FL, USA.
Institute for Food Safety and Health, Illinois Institute of Technology, Bedford Park, IL, USA.
J Sci Food Agric. 2018 Oct;98(13):4876-4884. doi: 10.1002/jsfa.9019. Epub 2018 May 14.
Muscadine grape pomace, a by-product of juicing and wine-making, contains significant amounts of anthocyanin 3,5-diglucosides, known to be beneficial to human health.
The objective of this research was to use mathematical modeling to investigate the adsorption/desorption characteristics of these anthocyanins from muscadine grape pomace on Amberlite FPX66 resin in a fixed bed column. Anthocyanins were extracted using hot water and ultrasound, and the extracts were loaded onto a resin column at five bed depths (5, 6, 8, 10 and 12 cm) using three flow rates (4, 6 and 8 mL min ). It was found that adsorption on the column fitted the bed depth service time (BDST) model and the empty bed residence time (EBRT) model. Desorption was achieved by eluting the column using ethanol at four concentrations (25, 40, 55 and 70% v/v) and could be described with an empirical sigmoid model. The breakthrough curves of anthocyanins fitted the BDST model for all three flow rates with R values of 0.983, 0.992 and 0.984 respectively. The EBRT model was successfully employed to find the operating lines, which allow for column scale-up while still achieving similar results to those found in a laboratory operation. Desorption with 40% (v/v) ethanol achieved the highest recovery rate of anthocyanins at 79.6%.
The mathematical models established in this study can be used in designing a pilot/industrial- scale column for the separation and concentration of anthocyanins from muscadine juice pomace. © 2018 Society of Chemical Industry.
麝香葡萄渣是榨汁和酿酒的副产品，含有大量的花翠素 3,5-二葡萄糖苷，已知对人体健康有益。
本研究的目的是使用数学模型来研究这些花色苷从麝香葡萄渣在固定床柱上的 Amberlite FPX66 树脂上的吸附/解吸特性。花色苷采用热水和超声提取，将提取物以五种床层深度（5、6、8、10 和 12 cm）和三种流速（4、6 和 8 mL min）加载到树脂柱上。结果表明，柱上的吸附符合床层深度服务时间（BDST）模型和空床保留时间（EBRT）模型。通过使用乙醇在四个浓度（25、40、55 和 70% v/v）洗脱柱来实现解吸，可以用经验的 S 型模型来描述。花色苷的穿透曲线适用于所有三种流速的 BDST 模型，R 值分别为 0.983、0.992 和 0.984。EBRT 模型成功地用于找到操作线，允许在柱规模放大的同时仍然获得与实验室操作中相似的结果。40%（v/v）乙醇的解吸可获得花色苷的最高回收率为 79.6%。
本研究建立的数学模型可用于设计从麝香葡萄汁渣中分离和浓缩花色苷的中试/工业规模柱。© 2018 化学工业协会。
