González Candela Rocío, Moverer Luciana, Calandra Ricardo Saúl, González-Calvar Silvia Inés, Vitullo Alfredo Daniel
Hidalgo 775,1405 Buenos Aires,Argentina.
Centro de Estudios Biomédicos,Biotecnológicos,Ambientales y Diagnóstico,Universidad Maimónides;Hidalgo 775,1405 Buenos Aires;Argentina.
Zygote. 2018 Apr;26(2):127-134. doi: 10.1017/S0967199418000023. Epub 2018 Mar 25.
SummaryThe Deleted in AZoospermia (DAZ) gene family regulates the development, maturation and maintenance of germ cells and spermatogenesis in mammals. The DAZ family consists of two autosomal genes, Boule and Dazl (Daz-like), and the Daz gene on chromosome Y. The aim of this study was to analyze the localization of DAZL and BOULE during testicular ontogeny of the seasonal-breeding Syrian hamster, Mesocricetus auratus. We also evaluated the testicular expression of DAZ family genes under short- or long-photoperiod conditions. In the pre-pubertal and adult testis, DAZL protein was found mainly in spermatogonia. BOULE was found in the spermatogonia from 20 days of age and during the pre-pubertal and adult period it was also detected in spermatocytes and round spermatids. DAZL and BOULE expression in spermatogonia was strictly nuclear only in 20-day-old hamsters. We also detected the novel mRNA and protein expression of BOULE in Leydig cells. In adult hamsters, Dazl expression was increased in regressed testis compared with non-regressed testis and DAZL protein expression was restricted to primary spermatocytes in regressed testis. These results show that DAZL and BOULE are expressed in spermatogonia at early stages in the Syrian hamster, then both proteins translocate to the cytoplasm when meiosis starts. In the adult regressed testis, the absence of DAZL in spermatogonia might be related to the decrease in germ cell number, suggesting that DAZ gene family expression is involved in changes in seminiferous epithelium during photoregression.
摘要
无精子症缺失基因（DAZ）家族调控哺乳动物生殖细胞的发育、成熟和维持以及精子发生。DAZ家族由两个常染色体基因，即Boule和Dazl（类Daz），以及Y染色体上的Daz基因组成。本研究的目的是分析季节性繁殖的叙利亚仓鼠（金仓鼠）睾丸个体发育过程中DAZL和BOULE的定位。我们还评估了短光照或长光照条件下DAZ家族基因在睾丸中的表达。在青春期前和成年睾丸中，DAZL蛋白主要存在于精原细胞中。BOULE在20日龄的精原细胞中被发现，在青春期前和成年期，它也在精母细胞和圆形精子细胞中被检测到。仅在20日龄的仓鼠中，精原细胞中的DAZL和BOULE表达严格定位于细胞核。我们还在睾丸间质细胞中检测到了BOULE新的mRNA和蛋白表达。在成年仓鼠中，与未退化的睾丸相比，退化睾丸中的Dazl表达增加，并且退化睾丸中的DAZL蛋白表达仅限于初级精母细胞。这些结果表明，在叙利亚仓鼠早期阶段，DAZL和BOULE在精原细胞中表达，然后在减数分裂开始时两种蛋白都转移到细胞质中。在成年退化睾丸中，精原细胞中DAZL的缺失可能与生殖细胞数量减少有关，这表明DAZ基因家族的表达参与了光周期消退期间生精上皮的变化。
