Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Eur J Pharm Sci. 2018 Jun 15;118:87-95. doi: 10.1016/j.ejps.2018.03.024. Epub 2018 Mar 21.
The aim of the present work was to develop a medication allowing for the combined delivery of platelet lysate (PL) and an anti-infective model drug, vancomycin hydrochloride (VCM), to chronic skin ulcers. A simple method was set up for the preparation of hyaluronic acid (HA) core-shell particles, loaded with PL and coated with calcium alginate, embedded in a VCM containing alginate matrix. Two different CaCl concentrations were investigated to allow for HA/PL core-shell particle formation. The resulting dressings were characterized for mechanical and hydration properties and tested in vitro (on fibroblasts) and ex-vivo (on skin biopsies) for biological activity. They were found of sufficient mechanical strength to withstand packaging and handling stress and able to absorb a high amount of wound exudate and to form a protective gel on the lesion area. The CaCl concentration used for shell formation did not affect VCM release from the alginate matrix, but strongly modified the release of PGFAB (chosen as representative of growth factors present in PL) from HA particles. In vitro and ex vivo tests provided sufficient proof of concept of the ability of dressings to improve skin ulcers healing.
本工作旨在开发一种药物，以实现血小板裂解液 (PL) 和抗感染模型药物盐酸万古霉素 (VCM) 的联合递送，用于慢性皮肤溃疡。建立了一种简单的方法，用于制备负载 PL 的透明质酸 (HA) 核壳粒子，并涂覆藻酸钠，嵌入含有藻酸钠基质的 VCM 中。研究了两种不同的 CaCl2 浓度以允许形成 HA/PL 核壳粒子。对所得敷料进行机械性能和水合性能进行了表征，并在体外（成纤维细胞）和体内（皮肤活检）进行了生物活性测试。结果表明，它们具有足够的机械强度，可以承受包装和处理过程中的压力，并且能够吸收大量伤口渗出物，并在病变区域形成保护性凝胶。用于壳形成的 CaCl2 浓度不影响 VCM 从藻酸钠基质中的释放，但强烈改变了 PGFAB（选择其作为 PL 中存在的生长因子的代表）从 HA 粒子中的释放。体外和体内测试充分证明了敷料改善皮肤溃疡愈合的能力。
