Department of Drug Science, University of Pavia, Pavia, Italy.
Department of Drug Science, University of Pavia, Pavia, Italy.
Int J Pharm. 2016 Jul 25;509(1-2):59-70. doi: 10.1016/j.ijpharm.2016.05.035. Epub 2016 May 20.
The aim of the present work was the development of a powder formulation for the delivery of manuka honey (MH) bioactive components and platelet lysate (PL) in chronic skin ulcers. In particular pectin (PEC)/chitosan (CS) particles were prepared by ionotropic gelation in the presence of calcium chloride and subsequently characterized for particle size, hydration properties and mechanical resistance. Different experimental conditions (calcium chloride and CS concentrations; rest time in the cationic solution) were considered in order to obtain particles characterized by optimal size, hydration properties and mechanical resistance. Two different fractions of MH were examined: one (Fr1), rich in methylglyoxal and the other (Fr2), rich in polyphenols. Particles were loaded with Fr1, fraction able to enhance in vitro proliferation of human fibroblasts, and with PL. The presence of CS in Fr1-loaded particles produced an improvement in cell proliferation. Moreover, PL loading into particles did not affect the biological activity of the hemoderivative. In vivo efficacy of PL- and Fr1-loaded particles was evaluated on a rat wound model. Both treatments markedly increased wound healing to the same extent.
本工作旨在开发一种粉末制剂，用于递送麦卢卡蜂蜜（MH）生物活性成分和血小板裂解液（PL）治疗慢性皮肤溃疡。具体而言，通过在氯化钙存在下的离子凝胶化制备了果胶（PEC）/壳聚糖（CS）颗粒，并对其粒径、水合性质和机械强度进行了表征。为了获得具有最佳粒径、水合性质和机械强度的颗粒，考虑了不同的实验条件（氯化钙和 CS 浓度；在阳离子溶液中的停留时间）。研究了两种不同的 MH 馏分：一种（Fr1）富含甲基乙二醛，另一种（Fr2）富含多酚。将 Fr1（能够增强人成纤维细胞体外增殖的馏分）和 PL 加载到颗粒中。CS 的存在使 Fr1 负载颗粒的细胞增殖能力得到提高。此外，PL 加载到颗粒中不会影响血液衍生物的生物学活性。PL 和 Fr1 负载颗粒的体内疗效在大鼠创面模型上进行了评估。两种治疗方法均能显著促进创面愈合，效果相当。
