Cunha Federico, Jeon Soo Jin, Daetz Rodolfo, Vieira-Neto Achilles, Laporta Jimena, Jeong K Casey, Barbet Anthony F, Risco Carlos A, Galvão Klibs N
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA; D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32610, USA.
Theriogenology. 2018 Jul 1;114:25-33. doi: 10.1016/j.theriogenology.2018.03.016. Epub 2018 Mar 16.
Metritis is caused by polymicrobial infection; however, recent metagenomic work challenges the importance of known pathogens such as Escherichia coli and Trueperella pyogenes while identifying potential new pathogens such as Bacteroides pyogenes, Porphyromonas levii and Helcococcus ovis. This study aims to quantify known and emerging uterine pathogens, and to evaluate their association with metritis and fever in dairy cows. Metritis was diagnosed at 6 ± 2 days postpartum, a uterine swab was collected and rectal temperature was measured. 39 cows were classified into three groups: Healthy (n = 14), Metritis without fever (MNoFever; n = 12), and Metritis with fever (MFever; n = 13). Absolute copy number was determined for total bacteria and for 8 potentially pathogenic bacteria using droplet digital PCR. Both MNoFever and MFever cows had higher copy number of total bacteria, Fusobacterium necrophorum, Prevotella melaninogenica, Bacteroides pyogenes, Porphyromonas levii, and Helcococcus ovis than Healthy cows. MNoFever and MFever groups were similar. There was no difference among groups in copy number of Escherichia coli, Trueperella pyogenes, and Bacteroides heparinolyticus, and they all had low copy numbers. Our work confirms the importance of some bacteria identified by culture-based studies in the pathogenesis of metritis such as Fusobacterium necrophorum and Prevotella melaninogenica; however, it challenges the importance of others such as Escherichia coli and Trueperella pyogenes at the time of metritis diagnosis. Additionally, Bacteroides pyogenes, Porphyromonas levii, and Helcococcus ovis were recognized as emerging pathogens involved in the etiology of metritis. Furthermore, fever was not associated with the total bacterial load or specific bacteria.
子宫炎由多种微生物感染引起；然而，最近的宏基因组学研究对已知病原体（如大肠杆菌和化脓放线杆菌）的重要性提出了挑战，同时鉴定出了潜在的新病原体，如化脓拟杆菌、产黑色素卟啉单胞菌和羊血球菌。本研究旨在量化已知和新出现的子宫病原体，并评估它们与奶牛子宫炎和发热的关联。在产后6±2天诊断子宫炎，采集子宫拭子并测量直肠温度。39头奶牛被分为三组：健康组（n = 14）、无发热子宫炎组（MNoFever；n = 12）和发热子宫炎组（MFever；n = 13）。使用液滴数字PCR测定总细菌和8种潜在致病细菌的绝对拷贝数。MNoFever组和MFever组奶牛的总细菌、坏死梭杆菌、产黑色素普雷沃菌、化脓拟杆菌、产黑色素卟啉单胞菌和羊血球菌的拷贝数均高于健康组奶牛。MNoFever组和MFever组相似。大肠杆菌、化脓放线杆菌和解肝素拟杆菌的拷贝数在各组间无差异，且它们的拷贝数均较低。我们的研究证实了一些基于培养的研究中鉴定出的细菌在子宫炎发病机制中的重要性，如坏死梭杆菌和产黑色素普雷沃菌；然而，它对其他细菌（如大肠杆菌和化脓放线杆菌）在子宫炎诊断时的重要性提出了挑战。此外，化脓拟杆菌、产黑色素卟啉单胞菌和羊血球菌被认为是参与子宫炎病因的新出现病原体。此外，发热与总细菌载量或特定细菌无关。
