Williamson K C, Tauber A I, Navarro J
J Leukoc Biol. 1987 Sep;42(3):239-44. doi: 10.1002/jlb.42.3.239.
The formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) dependent Ca2+ uptake by human neutrophils consists of at least two components, one of which is sensitive to dihydropyridine derivatives. Inhibition by dihydropyridine derivatives showed the rank order of nisoldepine greater than nitrendipine greater than nimodepine greater than/Bay K 8644. The nisoldepine-sensitive calcium uptake exhibited an ID50 of 1.5 microM and maximal inhibition were observed at 5 microM. Neither calcium efflux or [3H]fMet-Leu-Phe binding was affected by nisoldepine up to 10 microM. The inhibition of nisoldepine was inversely proportional to the extracellular calcium concentration. Unlabeled nisoldepine and other dihydropyridine derivatives displaced the specific binding of [3H]PN 200-110 to human neutrophils. Our data suggest a relationship between dihydropyridine binding and the inhibition of fMet-Leu-Phe-dependent Ca2+ uptake.
人中性粒细胞对甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMet-Leu-Phe）的依赖性钙离子摄取至少由两个部分组成，其中一个部分对二氢吡啶衍生物敏感。二氢吡啶衍生物的抑制作用呈现出尼索地平大于尼群地平大于尼莫地平大于/大于Bay K 8644的顺序。对尼索地平敏感的钙离子摄取表现出1.5微摩尔的半数抑制浓度（ID50），在5微摩尔时观察到最大抑制作用。高达10微摩尔的尼索地平对钙离子外流或[3H]fMet-Leu-Phe结合均无影响。尼索地平的抑制作用与细胞外钙离子浓度成反比。未标记的尼索地平和其他二氢吡啶衍生物取代了[3H]PN 200-110与人中性粒细胞的特异性结合。我们的数据表明二氢吡啶结合与fMet-Leu-Phe依赖性钙离子摄取的抑制之间存在关联。
