Agusti Montolio Susana, Cuenca Valera Rafaela, Lavín González Santiago, Cray Carolyn, Molina López Rafael, Ferron Emmanuel Serrano, Francisco Olga Nicolás, Marco Sánchez Ignasi, Casas-Díaz Encarna
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, FL, USA.
Vet Clin Pathol. 2018 Mar;47(1):78-93. doi: 10.1111/vcp.12559.
Blood biochemistry and hematology are essential in the laboratory diagnosis of disease. In Strigiformes, little information regarding reference values and influence of different preanalytic factors is available, while age is known to have an effect on some biochemistry analytes, especially in early life characterized by a rapid growth rate and increase in body mass.
The objective of this study was to determine baseline data for 29 blood biochemistry variables in 5 species of Iberian Strigiformes assigned to different age classes.
Healthy nocturnal birds living in wildlife health centers of Catalonia, Northeastern Spain were assigned to different age classes (chicks, juveniles, adults where possible and available) and their blood was collected and analyzed for different standard biochemistry variables. Species included Tawny owls, Little owls, Long-eared owls, Scop owls, and Barn owls.
A total of 276 clinically healthy animals were sampled, including between 179 chicks, 52 juveniles, and 45 adults. The RIs of cholesterol, albumin, and osmolality were the only variables that did not show interspecific variability. Common trends between age class groups were observed in 4 species. Phosphorus and calcium concentrations and ALP activities decreased with increasing age, while concentrations of sodium and γ-globulins tended to increase in the Strigidae family. Differences were concentrated in chicks and no differences were observed between juveniles and adults in any variable.
Our results demonstrate the importance of defining biochemical RIs specific for certain age classes.
血液生物化学和血液学在疾病的实验室诊断中至关重要。在鸮形目中，关于参考值和不同分析前因素影响的信息很少，而年龄已知会对一些生物化学分析物产生影响，尤其是在以快速生长率和体重增加为特征的早期生命阶段。
本研究的目的是确定5种伊比利亚鸮形目不同年龄组的29种血液生物化学变量的基线数据。
将生活在西班牙东北部加泰罗尼亚野生动物健康中心的健康夜行性鸟类分为不同年龄组（雏鸟、幼鸟、尽可能多的成年鸟），采集它们的血液并分析不同的标准生物化学变量。物种包括仓鸮、长耳鸮、短耳鸮、纵纹腹小鸮和灰林鸮。
总共对276只临床健康动物进行了采样，其中包括179只雏鸟、52只幼鸟和45只成年鸟。胆固醇、白蛋白和渗透压的参考区间是仅有的未表现出种间差异的变量。在4个物种中观察到了年龄组之间的共同趋势。磷、钙浓度和碱性磷酸酶活性随年龄增长而降低，而钠和γ球蛋白浓度在草鸮科中趋于升高。差异集中在雏鸟中，在任何变量中，幼鸟和成年鸟之间均未观察到差异。
我们的结果证明了定义特定年龄组生化参考区间的重要性。
