Stasch J P, Neuser D, John A, Hein D, Heuck K, Morich F J
Klin Wochenschr. 1987 Jul 15;65(12):533-7. doi: 10.1007/BF01727617.
A monoclonal antibody (mab) directed against alpha-human atrial natriuretic peptides (alpha-hANP) was produced. Using this mab a radioimmunoassay for the determination of hANP-like immunoreactivity in human plasma was established. To demonstrate the possible application of this radioimmunoassay for clinical diagnosis, plasma levels were measured in healthy subjects and in patients with renal failure before and after hemofiltration or hemodialysis. Plasma levels in healthy subjects ranged between less than 30 and 124 pg/ml. Mean plasma concentrations of hANP-IR in uremic subjects were 324 pg/ml before and 113 pg/ml after hemofiltration or hemodialysis.
制备了一种针对α-人心房利钠肽（α-hANP）的单克隆抗体（mab）。利用这种单克隆抗体建立了一种用于测定人血浆中hANP样免疫反应性的放射免疫分析法。为了证明这种放射免疫分析法在临床诊断中的可能应用，对健康受试者以及肾衰竭患者在血液滤过或血液透析前后的血浆水平进行了测量。健康受试者的血浆水平在低于30至124 pg/ml之间。尿毒症患者在血液滤过或血液透析前hANP-IR的平均血浆浓度为324 pg/ml，透析后为113 pg/ml。
