Lanzafame R J, Rogers D W, Naim J O, Herrera H R, Hinshaw J R
Lasers Surg Med. 1987;7(3):280-2. doi: 10.1002/lsm.1900070314.
This study reports the effect of hematoporphyrin derivative 2 (HPD2) on estrogen receptors (ER) in the animal model used to develop the clinical ER assay. Fifty 200-g female Sprague-Dawley rats were given 20 mg of dimethylbenzanthracene by gastric intubation. Spontaneous mammary tumors occurred in 35 animals. Animals were anesthetized and 50% of each tumor was removed when the tumors were 2 cm in diameter. Animals were then randomized to receive 5 mg/kg (group A), 10 mg/kg (group B), or 0 mg/kg (group C) HPD2 intravenously 48 hours after biopsy. The remaining tumor was excised 48 hours post HPD2 injection. All samples were weighed, placed on ice, and frozen to -70 degrees C. ER assay was performed by batch run. Results (fmol/mg cytosol) were as follows: All animals (n = 35) pre HPD2 34.2 +/- 5.4, post HPD2 34.2 +/- 5.8; group A: (n = 11) pre HPD2 33.9 +/- 7.9, post HPD2 37.1 +/- 7.8; group B: (n = 13) pre HPD2 29.2 +/- 3.8, post HPD2 25.5 +/- 3.6; group C: (n = 11) pre HPD2 40.3 +/- 5.2, post HPD2 41.5 +/- 7.9. HPD2 does not affect ER in this animal model. Confirmatory studies with human tumor material must be completed.
本研究报告了血卟啉衍生物2（HPD2）对用于开发临床雌激素受体（ER）检测方法的动物模型中雌激素受体的影响。50只体重200克的雌性斯普拉格-道利大鼠经胃管给予20毫克二甲基苯并蒽。35只动物发生了自发性乳腺肿瘤。当肿瘤直径达到2厘米时，将动物麻醉并切除每个肿瘤的50%。然后将动物随机分为三组，在活检后48小时分别静脉注射5毫克/千克（A组）、10毫克/千克（B组）或0毫克/千克（C组）的HPD2。在注射HPD2 48小时后切除剩余的肿瘤。所有样本称重后置于冰上，并冷冻至-70摄氏度。通过批量检测进行ER检测。结果（每毫克胞浆中的飞摩尔数）如下：所有动物（n = 35）在注射HPD2前为34.2±5.4，注射后为34.2±5.8；A组：（n = 11）注射HPD2前为33.9±7.9，注射后为37.1±7.8；B组：（n = 13）注射HPD2前为29.2±3.8，注射后为25.5±3.6；C组：（n = 11）注射HPD2前为40.3±5.2，注射后为41.5±7.9。在该动物模型中，HPD2不影响ER。必须完成用人肿瘤材料进行的验证性研究。
